 |
 |
|||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
||
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
|||||||||||||||||
 |
 |
 |
|
|||||||||||||||||||||||||||||||
Catalog Number: 10-5942-xx
Description: BHQ-2-dT
| 5'-Dimethoxytrityloxy-5-[(N-4''-carboxyethyl-4''-(N-ethyl)-4'-(4-Nitro-phenyldiazo)- 2'-methoxy-4'-methoxy-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C73H87N12O15P | M.W.: 1403.53 | F.W.: 962.91 |
Diluent: DCM:Anhydrous Acetonitrile (3:1) |
| Coupling: 15 minute coupling time recommended |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged. |
| Catalog Information | Material Safety Data Sheet |
Frequently Asked Technical Question
QUESTION: What are the physical properties of the various black hole quenchers?
RESPONSE:
| Quencher | lmax (nm) | E260 (L/mol.cm) | Emax (L/mol.cm) |
| BHQ-0 | 493 | 7,700 | 34,000 |
| BHQ-1 | 534 | 8,000 | 34,000 |
| BHQ-2 | 579 | 8,000 | 38,000 |
| BHQ-3 | 672 | 13,000 | 42,700 |
REFERENCE(S):
Glen Report 17.1
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 1.78 | 46 | 27.6 | 17.25 | 12.55 | 9.2 | 2.3 |
| 10-5942-90 | 100µmoles | .14grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5942-95 | 50µmoles | .07grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 2.66 | .067 | 46.8 | 29.25 | 21.27 | 2.93 | |
| 10-5942-90 | 100µmoles | .14grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5942-95 | 50µmoles | .07grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 2.66 | .067 | 48.4 | 30.25 | 22 | ||
| 10-5942-90 | 100µmoles | .14grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-5942-95 | 50µmoles | .07grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-5942.html