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Catalog Number: 10-5920-xx
Description: Epoch Redmond Red™Phosphoramidite
| 3-(3-oxo-7-pivaloylphenoxazin-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)- pyrrolidin-4-yl-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C55H63N4O10P | M.W.: 971.09 | F.W.: 445.34 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx) |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: |
| Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc. |
| Please see Terms and Conditions of Use |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 15.1: Redmond red (TM) And yakima yellow (TM) dyes, and eclipse (TM) non-fluorescent quencher |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5920-02 | 0.25grams | .25grams | 2.57 | 72.33 | 43.4 | 27.13 | 19.73 | 14.47 | 3.62 |
| 10-5920-90 | 100µmoles | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5920-95 | 50µmoles | .049grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5920-02 | 0.25grams | .25grams | 3.84 | .07 | 70.4 | 44 | 32 | 4.4 | |
| 10-5920-90 | 100µmoles | .097grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5920-95 | 50µmoles | .049grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5920-02 | 0.25grams | .25grams | 3.84 | .07 | 72 | 45 | 32.73 | ||
| 10-5920-90 | 100µmoles | .097grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5920-95 | 50µmoles | .049grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-5920.html