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Catalog Number: 10-5918-xx
Description: DyLight DY647 Phosphoramidite
| 1,1'-dimethyl-3,3,3'-trimethyl-3'-(5-[(2-cyanoethyl)-(N,N-diisopropyl)- phosphoramidityl]pentyl)-indodicarbocyanine tosylate | ||
| Formula: C47H63N4O5PS | M.W.: 827.07 | F.W.: 516.61 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. The use of UltraMILD monomers are preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). 0.02M Iodine for oxidation is recommended. |
| Deprotection: If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at Room Temperature in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields. However, the oligonucleotide will require a reverse phase purification, e.g. PolyPak. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 21.1: NEW FLUORESCENT PHOSPHORAMIDITES - SIMA (HEX), DYLIGHT™ |
| Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5918-95 | 50µmoles | .041grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-5918-90 | 100µmoles | .083grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5918-02 | 0.25grams | .25grams | 3.02 | 87.33 | 52.4 | 32.75 | 23.82 | 17.47 | 4.37 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5918-95 | 50µmoles | .041grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-5918-90 | 100µmoles | .083grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5918-02 | 0.25grams | .25grams | 4.51 | .07 | 83.8 | 52.38 | 38.09 | 5.24 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5918-95 | 50µmoles | .041grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-5918-90 | 100µmoles | .083grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5918-02 | 0.25grams | .25grams | 4.51 | .07 | 85.4 | 53.38 | 38.82 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-5918.html