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Catalog Number: 10-4950-xx
Description: PC Biotin Phosphoramidite
| 1-[2-Nitro-5-(6-(N-(4,4'-dimethoxytrityl))-biotinamidocaproamidomethyl)phenyl]- ethyl-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C55H72N7O9PS | M.W.: 1038.25 | F.W.: 597.62 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 2 minute coupling time recommended |
| Deprotection: PC-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 14.1: PC Biotin and Related Photocleavable Modifiers |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 2.41 | 67 | 40.2 | 25.13 | 18.27 | 13.4 | 3.35 |
| 10-4950-90 | 100µmoles | .104grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-4950-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 3.59 | .07 | 65.4 | 40.88 | 29.73 | 4.09 | |
| 10-4950-90 | 100µmoles | .104grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-4950-95 | 50µmoles | .052grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 3.59 | .07 | 67 | 41.88 | 30.45 | ||
| 10-4950-90 | 100µmoles | .104grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-4950-95 | 50µmoles | .052grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-4950.html