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Catalog Number: 10-3501-xx
Description: 1-Me-A-CE Phosphoramidite
| 5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-Adenosine, 2'-O-TBDMS- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H63ClN7O7PSi | M.W.: 944.57 | F.W.: 344.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling time recommended. Monomers that allow for UltraMILD deprotection are recommended. (Pac-A:10-3000-xx, Ac-C: 10-3015-xx, iPr-Pac-G: 10-3021-xx, U: 10-3030-xx ). |
| Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using amidites with standard protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 19.1: 1-Methyl-Adenine in nucleic acids |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-3501-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3501-02 | 0.25grams | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 72.6 | 45.38 | 33 | 4.54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 74.2 | 46.38 | 33.73 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-3501.html