10-3190, 2'-OMe-5-Br-U-CE Phosphoramidite Glen Research Corporation products for Minor Base of DNA and RNA

Catalog Number: 10-3190-xx

Description: 2'-OMe-5-Br-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-bromo-Uridine,2'-O-methyl,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite
Formula: C₄₀H₄₈BrN₄O₉P	M.W.: 839.72	F.W.: 399.09

Diluent: Acetonitrile:THF (1:1)

Coupling: Dissolve in Acetonitrile:THF (1:1).A 6 minute coupling time is recommended.

Deprotection: Mild deprotection: Ammonium Hyrdoxide for 24 hours at room temperature.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

Catalog Information

Material Safety Data Sheet

Literature Highlights

Glen Report 11.2: MORE NOVEL MONOMERS

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	Pack Size	Grams/ Pack	0.1M Dil. (mL)	Approximate Number of Additions
10-3190-02	0.25grams	.25grams	2.98	86	51.6	32.25	23.45	17.2	4.3
10-3190-90	100µmoles	.084grams	1	20	12	7.5	5.45	4	1
Expedite
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Approximate Number of Additions
10-3190-02	0.25grams	.25grams	4.44	.07	82.4	51.5	37.45	5.15
10-3190-90	100µmoles	.084grams	1.5	.07	23.6	14.75	10.73	1.48
Beckman
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Molarity	30nm	200nm	1000nm
Cat.No.	Pack Size	Grams/ Pack	Dilution (mL)	Approximate Number of Additions
10-3190-02	0.25grams	.25grams	4.44	.07	84	52.5	38.18
10-3190-90	100µmoles	.084grams	1.5	.07	25.2	15.75	11.45

11/04/2011 | http://www.glenres.com/ProductFiles/10-3190.html