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Catalog Number: 10-3072-xx
Description: 6-Thio-G-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C53H71N8O8PSSi | M.W.: 1039.31 | F.W.: 361.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015). |
| Deprotaction: Treat column with 1 M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50 mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection Ð see Technical Bulletin. (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf) Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 17.1: Products for siRNA Research |
| Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 2.41 | 67 | 40.2 | 25.13 | 18.27 | 13.4 | 3.35 |
| 10-3072-90 | 100µmoles | .104grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3072-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 3.59 | .067 | N/A | N/A | 13.63 | N/A | |
| 10-3072-90 | 100µmoles | .104grams | 1.5 | .067 | N/A | N/A | 4.92 | N/A | |
| 10-3072-95 | 50µmoles | .052grams | .75 | .067 | N/A | N/A | 1.79 | N/A | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3072-02 | 0.25grams | .25grams | 3.59 | .067 | N/A | 10.81 | 10.15 | ||
| 10-3072-90 | 100µmoles | .104grams | 1.5 | .067 | N/A | 4.06 | 3.82 | ||
| 10-3072-95 | 50µmoles | .052grams | .75 | .067 | N/A | 1.65 | 1.55 | ||
06/25/2010 | http://www.glenres.com/ProductFiles/10-3072.html