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Catalog Number: 10-3050-xx
Description: 5-Me-U-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-Methyl-Uridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C46H63N4O9PSi | M.W.: 875.08 | F.W.: 320.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time recommended |
| Deprotection: RNA deprotection. See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-3050 | 402 |
Literature Highlights
| Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3050-02 | 0.25grams | .25grams | 2.86 | 82 | 49.2 | 30.75 | 22.36 | 16.4 | 4.1 |
| 10-3050-90 | 100µmoles | .088grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3050-95 | 50µmoles | .044grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3050-02 | 0.25grams | .25grams | 4.26 | .07 | N/A | N/A | 16.42 | N/A | |
| 10-3050-90 | 100µmoles | .088grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
| 10-3050-95 | 50µmoles | .044grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3050-02 | 0.25grams | .25grams | 4.26 | .07 | N/A | 12.97 | 12.18 | ||
| 10-3050-90 | 100µmoles | .088grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
| 10-3050-95 | 50µmoles | .044grams | .75 | .07 | N/A | 1.65 | 1.55 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-3050.html