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Catalog Number: 10-3017-xx
Description: Pyrrolo-C-TOM-CE Phosphoramidite
| 5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]- 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C52H72N5O9PSi | M.W.: 970.23 | F.W.: 343.27 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended |
| Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 16.1: Minor Base and Related Novel Phosphoramidites |
| Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3017-02 | 0.25grams | .25grams | 2.58 | 72.67 | 43.6 | 27.25 | 19.82 | 14.53 | 3.63 |
| 10-3017-90 | 100µmoles | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3017-95 | 50µmoles | .049grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3017-02 | 0.25grams | .25grams | 3.85 | .07 | N/A | N/A | 14.71 | N/A | |
| 10-3017-90 | 100µmoles | .097grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
| 10-3017-95 | 50µmoles | .049grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3017-02 | 0.25grams | .25grams | 3.85 | .07 | N/A | 11.65 | 10.94 | ||
| 10-3017-90 | 100µmoles | .097grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
| 10-3017-95 | 50µmoles | .049grams | .75 | .07 | N/A | 1.65 | 1.55 | ||
01/18/2013 | http://www.glenres.com/ProductFiles/10-3017.html