 |
 |
|||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
||
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||||
Catalog Number: 10-3000-xx
Description: Pac-A-CE Phosphoramidite
| 5'-Dimethoxytrityl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'- [(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C54H68N7O9PSi | M.W.: 1018.23 | F.W.: 329.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: RNA Deprotection-see bulletin Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3000-02 | 0.25grams | .25grams | 2.46 | 68.67 | 41.2 | 25.75 | 18.73 | 13.73 | 3.43 |
| 10-3000-05 | 0.5grams | .5grams | 4.91 | 150.33 | 90.2 | 56.38 | 41 | 30.07 | 7.52 |
| 10-3000-10 | 1.0gram | 1grams | 9.82 | 314 | 188.4 | 117.75 | 85.64 | 62.8 | 15.7 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3000-02 | 0.25grams | .25grams | 3.66 | .07 | N/A | N/A | 13.92 | N/A | |
| 10-3000-05 | 0.5grams | .5grams | 7.33 | .07 | N/A | N/A | 29.21 | N/A | |
| 10-3000-10 | 1.0gram | 1grams | 14.66 | .07 | N/A | N/A | 59.75 | N/A | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3000-02 | 0.25grams | .25grams | 3.66 | .07 | N/A | 11.03 | 10.36 | ||
| 10-3000-05 | 0.5grams | .5grams | 7.33 | .07 | N/A | 22.87 | 21.48 | ||
| 10-3000-10 | 1.0gram | 1grams | 14.66 | .07 | N/A | 46.52 | 43.7 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-3000.html