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Catalog Number: 10-1995-xx
Description: Protected BiotinLC Serinol Phosphoramidite
| 3-Dimethoxytrityloxy-2-(3-((4-t-butylbenzoyl)-biotinyl-3-aminopropyl)- diethyleneglycolyl-propylamido-glycanoylamido)propyl-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C68H96N7O14PS | M.W.: 1298.57 | F.W.: 697.74 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12-15 minute coupling time recommended |
| Deprotection: Synthesize using acetyl-protected dC and deprotect in 30% ammonia/40% methylamine 1:1 (AMA) at 65°C for 10 minutes. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 20.2: Novel Reagents for Modification and Labelling |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1995-02 | 0.25grams | .25grams | 1.93 | 51 | 30.6 | 19.13 | 13.91 | 10.2 | 2.55 |
| 10-1995-90 | 100µmoles | .13grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1995-95 | 50µmoles | .065grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1995-02 | 0.25grams | .25grams | 2.87 | .07 | 51 | 31.88 | 23.18 | 3.19 | |
| 10-1995-90 | 100µmoles | .13grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1995-95 | 50µmoles | .065grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1995-02 | 0.25grams | .25grams | 2.87 | .07 | 52.6 | 32.88 | 23.91 | ||
| 10-1995-90 | 100µmoles | .13grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1995-95 | 50µmoles | .065grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-1995.html