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Catalog Number: 10-1964-xx
Description: 6-Fluorescein Phosphoramidite
| 1-Dimethoxytrityloxy-2-(6-carboxy-(di-O-pivaloyl-fluorescein)-4-aminobutyl)-propyl-3-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C68H78N3O13P | M.W.: 1176.35 | F.W.: 566.48 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling time recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 13.1: NEW MONOMERS: 2,4-DIFLUOROTOLUENE, FLUORESCEIN PHOSPHORAMIDITE AND SUPPORT, 2',3'-DIDEOXYNUCLEOSIDES |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:
| Dye | E 260 nm | E lambda max | Excitation max | Emission max | QY | Notes |
| (L/mol cm) | (L/mol cm) | (nm) | (nm) | |||
| 6-FAM | 21,000 | 75,000 | 494 | 520 | 0.9 | |
| Fluorescein-dT | 38,800 | 75,000 | 494 | 522 | 0.9 | |
| TET | 16,300 | 86,000 | 521 | 541 | 0.9 | |
| HEX | 31,600 | 96,000 | 535 | 553 | 0.7 | |
| TAMRA | 32,300 | 89,000 | 556 | 580 | 0.7 | |
| Cy3 | 5,000 | 150,000 | 546 | 563 | 0.1 | |
| Cy3.5 | 24,000 | 116,000 | 591 | 604 | 0.35 | |
| Cy5 | 10,000 | 250,000 | 646 | 662 | 0.2 | |
| Cy5.5 | 21,500 | 209,000 | 688 | 707 | ||
| acridine | 39,500 | 9,120 | 421 | 497 | ||
| NBD | 3,700 | 19,500 | 485 | 535 | 0.1 | |
| Etheno-dA | 4,800 | 5,800 | 276 | 405 | 0.035 | |
| Furano-dT | 4,000 | 3,700 | 345 | 470 | 0.07/0.02 | QY 0.07 single-stranded; 0.02 ds, deprotected in ammonia 55°C ON |
| 2-aminopurine | 1,000 | 3,600 | 303 | 371 |
REFERENCE(S):
ABI User Bulletin #78, ABI website; http://www2.perkin-elmer.com:80/ds/239622/239622.html, Oligonucleotide Properties Calculator; http://www.basic.nwu.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1964-02 | 0.25grams | .25grams | 2.13 | 57.67 | 34.6 | 21.63 | 15.73 | 11.53 | 2.88 |
| 10-1964-90 | 100µmoles | .118grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1964-92 | 25µmoles | .029grams | .25 | -5 | -3 | -1.88 | -1.36 | -1 | -.25 |
| 10-1964-95 | 50µmoles | .059grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1964-02 | 0.25grams | .25grams | 3.17 | .067 | 57 | 35.63 | 25.91 | 3.56 | |
| 10-1964-90 | 100µmoles | .118grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1964-92 | 25µmoles | .029grams | .375 | .067 | 1.2 | .75 | .55 | .08 | |
| 10-1964-95 | 50µmoles | .059grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1964-02 | 0.25grams | .25grams | 3.17 | .067 | 58.6 | 36.63 | 26.64 | ||
| 10-1964-90 | 100µmoles | .118grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1964-92 | 25µmoles | .029grams | .375 | .067 | 2.8 | 1.75 | 1.27 | ||
| 10-1964-95 | 50µmoles | .059grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1964.html