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Catalog Number: 10-1925-xx
Description: Long Trebler Phosphoramidite
| Tris-2,2,2-[3-(4,4'-dimethoxytrityloxy)propyloxymethyl]methyleneoxypropyl- [(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C89H107N2O15P | M.W.: 1475.78 | F.W.: 428.41 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time using DCI activator (30-3150-xx) is recommended for the trebler addition. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 19.1: Microarrays, Nanotechnology and Beyond |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1925-02 | 0.25grams | .25grams | 1.69 | 43 | 25.8 | 16.13 | 11.73 | 8.6 | 2.15 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .07 | 44.2 | 27.63 | 20.09 | 2.76 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .07 | 45.8 | 28.63 | 20.82 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-1925.html