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Catalog Number: 10-1904-xx
Description: Fluorous Chemical Phosphorylation Reagent II
| [3-(Fluorous-dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite | ||
| Formula: C49H54F17N2O9P | M.W.: 1168.92 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 6 minute coupling time. Omit the capping step after the addition of this reagent! |
| Deprotection: See Technical Bulletin for details (Technical Bulletin). |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| Please Note: This product is covered by US Patent No.: 5,959,090. Products for Fluorous Affinity Purification of Oligonucleotides: Patent pending, Berry & Associates, Inc. The use of these products is licensed under U.S. Patents 6,673,539, 6,156,896; 5,859,247; and 5,777,121 and one or more pending patents owned or controlled by Fluorous Technologies, Inc. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 18.1: Fluorous Affinity Purification of Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1904-02 | 0.25grams | .25grams | 2.14 | 58 | 34.8 | 21.75 | 15.82 | 11.6 | 2.9 |
| 10-1904-90 | 100µmoles | .117grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1904-02 | 0.25grams | .25grams | 3.19 | .07 | 57.4 | 35.88 | 26.09 | 3.59 | |
| 10-1904-90 | 100µmoles | .117grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1904-02 | 0.25grams | .25grams | 3.19 | .07 | 59 | 36.88 | 26.82 | ||
| 10-1904-90 | 100µmoles | .117grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
01/15/2013 | http://www.glenres.com/ProductFiles/10-1904.html