 |
 |
|||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
||
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||||
Catalog Number: 10-1902-xx
Description: Solid Chemical Phosphorylation Reagent II
| [3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C37H49N4O7P | M.W.: 692.79 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5' Phosphate. If the DMT is retained, see Technical Bulletin for removal procedure (www.glenresearch.com/Technical/TB_CPR_II.pdf). Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: No data available |
| Please Note: Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090 and European Patent: EP0816368 |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 20.1: New Products |
| Glen Report 23.1: Technical Brief - Chemical Phosphorylation Ð considering the options |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1902-02 | 0.25grams | .25grams | 3.61 | 107 | 64.2 | 40.13 | 29.18 | 21.4 | 5.35 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .07 | 101.4 | 63.38 | 46.09 | 6.34 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .07 | 103 | 64.38 | 46.82 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-1902.html