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Catalog Number: 10-1901-xx

Description: Chemical Phosphorylation Reagent II

[3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)-
(N,N-diisopropyl)-phosphoramidite
Formula: C39H51N2O9PM.W.: 722.82F.W.: 79.98

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: 6 minute coupling time. Omit the capping step after the addition of this reagent!
Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the CPR II to yield a 5' Phosphate. If the DMT is retained, see Technical Bulletin for removal procedure (www.glenresearch.com/Technical/TB_CPR_II.pdf). Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours
Please Note: Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090 and European Patent: EP0816368
Catalog InformationMaterial Safety Data Sheet

Literature Highlights

Glen Report 8.2: 5'-PHOSPHORYLATION - NOW COMPATIBLE WITH DMT-ON PURIFICATION
Glen Report 12.1: PRODUCT UPDATE - WHICH CHEMICAL PHOSPHORYLATION REAGENT?
Glen Report 20.1: New Products
Glen Report 23.1: Technical Brief - Chemical Phosphorylation Ð considering the options

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1901-020.25grams.25grams3.4610261.238.2527.8220.45.1
10-1901-90100µmoles.072grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1901-020.25grams.25grams5.16.0796.860.5446.05
10-1901-90100µmoles.072grams1.5.0723.614.7510.731.48
Beckman
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity30nm200nm1000nm
Approximate Number of Additions
10-1901-020.25grams.25grams5.16.0798.461.544.73
10-1901-90100µmoles.072grams1.5.0725.215.7511.45

11/04/2011 | http://www.glenres.com/ProductFiles/10-1901.html