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Catalog Number: 10-1901-xx
Description: Chemical Phosphorylation Reagent II
| [3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite | ||
| Formula: C39H51N2O9P | M.W.: 722.82 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf. |
| Coupling: 6 minute coupling time. Omit the capping step after the addition of this reagent! |
| Deprotection: SEE USERS GUIDE TO DNA MODIFICATION Deprotection Detail |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 24 hours |
| Please Note: This product is covered by US Patent No.: 5,959,090. |
| Catalog Information | Material Safety Data Sheet | Technical Bulletin |
Literature Highlights
| Glen Report 8.2: 5'-PHOSPHORYLATION - NOW COMPATIBLE WITH DMT-ON PURIFICATION |
| Glen Report 12.1: PRODUCT UPDATE - WHICH CHEMICAL PHOSPHORYLATION REAGENT? |
| Glen Report 20.1: New Products |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1901-02 | 0.25grams | .25grams | 3.46 | 102 | 61.2 | 38.25 | 27.82 | 20.4 | 5.1 |
| 10-1901-90 | 100µmoles | .072grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1901-02 | 0.25grams | .25grams | 5.16 | .067 | 96.8 | 60.5 | 44 | 6.05 | |
| 10-1901-90 | 100µmoles | .072grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1901-02 | 0.25grams | .25grams | 5.16 | .067 | 98.4 | 61.5 | 44.73 | ||
| 10-1901-90 | 100µmoles | .072grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1901.html