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Catalog Number: 10-1900-xx
Description: Chemical Phosphorylation Reagent
| 2-[2-(4,4'-Dimethoxytrityloxy)ethylsulfonyl]ethyl-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite | ||
| Formula: C34H45N2O7PS | M.W.: 656.77 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification. Deprotection Detail |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is provided under license from Bayer Corporation. |
| The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification The user may remove the DMT during synthesis to check coupling efficiency. This reagent can be used to create the 3Õ-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis-Both the support base and the CPR are cleaved |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 8.2: 5'-PHOSPHORYLATION - NOW COMPATIBLE WITH DMT-ON PURIFICATION |
| Glen Report 12.1: PRODUCT UPDATE - WHICH CHEMICAL PHOSPHORYLATION REAGENT? |
| Glen Report 20.1: New Products |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 3.81 | 113.67 | 68.2 | 42.63 | 31 | 22.73 | 5.68 |
| 10-1900-90 | 100µmoles | .066grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1900-95 | 50µmoles | .033grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .067 | 107.2 | 67 | 48.73 | 6.7 | |
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1900-95 | 50µmoles | .033grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .067 | 108.8 | 68 | 49.45 | ||
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1900-95 | 50µmoles | .033grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1900.html