 |
 |
|||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
||
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||||
Catalog Number: 10-1621-xx
Description: iPr-Pac-dG-CE Phosphoramidite
| 5'-Dimethoxytrityl-N-p-isopropyl-phenoxyacetyl-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H60N7O9P | M.W.: 946.05 | F.W.: 329.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.2: ALTERNATIVES TO EXPEDITE MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1621-02 | 0.25grams | .25grams | 2.64 | 74.67 | 44.8 | 28 | 20.36 | 14.93 | 3.73 |
| 10-1621-05 | 0.5grams | .5grams | 5.29 | 163 | 97.8 | 61.13 | 44.45 | 32.6 | 8.15 |
| 10-1621-10 | 1.0gram | 1grams | 10.57 | 339 | 203.4 | 127.13 | 92.45 | 67.8 | 16.95 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1621-02 | 0.25grams | .25grams | 3.94 | .07 | 72.4 | 45.25 | 32.91 | 4.53 | |
| 10-1621-05 | 0.5grams | .5grams | 7.89 | .07 | 151.4 | 94.63 | 68.82 | 9.46 | |
| 10-1621-10 | 1.0gram | 1grams | 15.78 | .07 | 309.2 | 193.25 | 140.55 | 19.33 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1621-02 | 0.25grams | .25grams | 3.94 | .07 | 74 | 46.25 | 33.64 | ||
| 10-1621-05 | 0.5grams | .5grams | 7.89 | .07 | 153 | 95.63 | 69.55 | ||
| 10-1621-10 | 1.0gram | 1grams | 15.78 | .07 | 310.8 | 194.25 | 141.27 | ||
12/11/2012 | http://www.glenres.com/ProductFiles/10-1621.html