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Catalog Number: 10-1590-xx
Description: Pyrene-dU-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-(pyren-1-yl-ethynyl)-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C57H55N4O8P | M.W.: 955.04 | F.W.: 514.42 |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v) |
| Coupling: 6 minute coupling time recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 19.2: Versatile New Reagents Ð Pyrene-dU and Perylene-dU |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1590-95 | 50µmoles | .048grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1590-90 | 100µmoles | .096grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1590-02 | 0.25grams | .25grams | 2.62 | 74 | 44.4 | 27.75 | 20.18 | 14.8 | 3.7 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1590-95 | 50µmoles | .048grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1590-90 | 100µmoles | .096grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1590-02 | 0.25grams | .25grams | 3.91 | .07 | 71.8 | 44.88 | 32.64 | 4.49 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1590-95 | 50µmoles | .048grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1590-90 | 100µmoles | .096grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1590-02 | 0.25grams | .25grams | 3.91 | .07 | 73.4 | 45.88 | 33.36 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1590.html