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Catalog Number: 10-1535-xx
Description: NHS-Carboxy-dT
| 5'-Dimethoxytrityl-5-[3-acrylate NHS ester]-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C46H52N5O12P | M.W.: 897.91 | F.W.: varies, -CO2H=360.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: See Glen Report 19.1 for details (www.glenres.com/GlenReports/GR19-1.pdf). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
| Catalog Information | Material Safety Data Sheet | Technical Bulletin |
Literature Highlights
| Glen Report 19.1: NHS-Carboxy-dT - Expanding the NHS Ester Phosphoramidite Repertoire |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1535-90 | 100µmoles | .09grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1535-02 | 0.25grams | .25grams | 2.78 | 79.33 | 47.6 | 29.75 | 21.64 | 15.87 | 3.97 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1535-90 | 100µmoles | .09grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1535-02 | 0.25grams | .25grams | 4.16 | .067 | 76.8 | 48 | 34.91 | 4.8 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1535-90 | 100µmoles | .09grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1535-02 | 0.25grams | .25grams | 4.16 | .067 | 78.4 | 49 | 35.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1535.html