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Catalog Number: 10-1532-xx
Description: 5'-Dabsyl-dT-CE Phosphoramidite
| 5'-Dabsyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C33H44N7O8PS | M.W.: 729.78 | F.W.: 591.53 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dGwith acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 19.1: Quenched Autoligation (QUAL) Probes |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1532-02 | 0.25grams | .25grams | 3.43 | 101 | 60.6 | 37.88 | 27.55 | 20.2 | 5.05 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1532-02 | 0.25grams | .25grams | 5.11 | .07 | 95.8 | 59.88 | 43.55 | 5.99 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1532-90 | 100µmoles | .073grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1532-02 | 0.25grams | .25grams | 5.11 | .07 | 97.4 | 60.88 | 44.27 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1532.html