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Catalog Number: 10-1470-xx
Description: CleanAmp™-dT-CE Phosphoramidite
| 5'-Dimethoxytrityl-2'-deoxyThymidine, 3'-[(4-oxo-tetradecyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H72N3O9P | M.W.: 902.11 | F.W.: FW: 514.55 (triester) |
Diluent: Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf. |
| Coupling: 10 minute coupling time. CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix. |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Glen Report 21.2 for details. (Technical Bulletin) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 21.1: HOT START PCR UPDATE: CLEANAMP™ PRIMERS |
| Glen Report 21.1: PROCEDURE FOR SYNTHESIS OF CLEANAMP™ PRIMERS |
| Glen Report 21.2: Purification of CleanAmp™ DNA Oligonucleotides (DMT-ON) |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1470-02 | 0.25grams | .25grams | 2.77 | 79 | 47.4 | 29.63 | 21.55 | 15.8 | 3.95 |
| 10-1470-05 | 0.5grams | .5grams | 5.54 | 171.33 | 102.8 | 64.25 | 46.73 | 34.27 | 8.57 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1470-02 | 0.25grams | .25grams | 4.14 | .07 | 76.4 | 47.75 | 34.73 | 4.78 | |
| 10-1470-05 | 0.5grams | .5grams | 8.27 | .07 | 159 | 99.38 | 72.27 | 9.94 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1470-90 | 100µmoles | .09grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1470-02 | 0.25grams | .25grams | 4.14 | .07 | 78 | 48.75 | 35.45 | ||
| 10-1470-05 | 0.5grams | .5grams | 8.27 | .07 | 160.6 | 100.38 | 73 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1470.html