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Catalog Number: 10-1420-xx
Description: F-DMT-dG-CE Phosphoramidite
| 5'-Fluorous-dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C54H57F17N7O8P | M.W.: 1286.03 | F.W.: 329.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Normal |
| Please Note: Products for Fluorous Affinity Purification of Oligonucleotides: Patent pending, Berry & Associates, Inc. The use of these products is licensed under U.S. Patents 6,673,539, 6,156,896; 5,859,247; and 5,777,121 and one or more pending patents owned or controlled by Fluorous Technologies, Inc. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 18.1: Fluorous Affinity Purification of Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1420-02 | 0.25grams | .25grams | 1.94 | 51.33 | 30.8 | 19.25 | 14 | 10.27 | 2.57 |
| 10-1420-90 | 100µmoles | .129grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1420-02 | 0.25grams | .25grams | 2.9 | .07 | 51.6 | 32.25 | 23.45 | 3.23 | |
| 10-1420-90 | 100µmoles | .129grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1420-02 | 0.25grams | .25grams | 2.9 | .07 | 53.2 | 33.25 | 24.18 | ||
| 10-1420-90 | 100µmoles | .129grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1420.html