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Catalog Number: 10-1321-xx
Description: iPr-Pac-dG-Me Phosphoramidite
| 5'-Dimethoxytrityl-N-p-isopropyl-phenoxyacetyl-Guanosine, 3'-[methyl-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C49H59N6O9P | M.W.: 907.01 | F.W.: 343.23 (Methyl triester) FW: 329.21 (Phosphodiester) |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use UltraMild Cap Mix A (40-4210-xx/40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature to leave the methyl phosphotriester intact. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 14.1: PRODUCT UPDATE - HOW ARE METHYL TRIESTER LINKAGES PREPARED? |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1321-02 | 0.25grams | .25grams | 2.76 | 78.67 | 47.2 | 29.5 | 21.45 | 15.73 | 3.93 |
| 10-1321-05 | 0.5grams | .5grams | 5.51 | 170.33 | 102.2 | 63.88 | 46.45 | 34.07 | 8.52 |
| 10-1321-10 | 1.0gram | 1grams | 11.03 | 354.33 | 212.6 | 132.88 | 96.64 | 70.87 | 17.72 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1321-02 | 0.25grams | .25grams | 4.11 | .07 | 75.8 | 47.38 | 34.45 | 4.74 | |
| 10-1321-05 | 0.5grams | .5grams | 8.23 | .07 | 158.2 | 98.88 | 71.91 | 9.89 | |
| 10-1321-10 | 1.0gram | 1grams | 16.46 | .07 | 322.8 | 201.75 | 146.73 | 20.18 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1321-02 | 0.25grams | .25grams | 4.11 | .07 | 77.4 | 48.38 | 35.18 | ||
| 10-1321-05 | 0.5grams | .5grams | 8.23 | .07 | 159.8 | 99.88 | 72.64 | ||
| 10-1321-10 | 1.0gram | 1grams | 16.46 | .07 | 324.4 | 202.75 | 147.45 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1321.html