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Catalog Number: 10-1210-xx
Description: dC-H-Phosphonate, DBU Salt
| 5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,3'-H-phosphonate, DBU salt | ||
| Formula: C46H52N5O9P | M.W.: 849.35 | F.W.: 289.18 |
Diluent: Acetonitrile/Pyridine (1:1) |
| Coupling: H-Phosphonate Synthesis Protocol.(The DBU is now being provided in place of the TEA salt for dC-H Phosphonate. This product shows better monomer stability and solubility.) |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: ~1week |
| Oxidation only need to be done at end of synthesis. |
| Catalog Information | Material Safety Data Sheet | Technical Bulletin |
Frequently Asked Technical Question
QUESTION: What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?
RESPONSE:1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.
2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)
3. Oxidation: Following synthesis
Step 1: React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes
Step 2: Remove solution from support
Step 3: React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes
Step 4: Flush column with acetonitrile and blow dry support
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1210-02 | 0.25grams | .25grams | 21 | 686.67 | 412 | 257.5 | 187.27 | 137.33 | 34.33 |
| 10-1210-05 | 0.5grams | .5grams | 42 | 1386.67 | 832 | 520 | 378.18 | 277.33 | 69.33 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1210-02 | 0.25grams | .25grams | 5 | .059 | 93.6 | 58.5 | 42.55 | 5.85 | |
| 10-1210-05 | 0.5grams | .5grams | 10 | .059 | 193.6 | 121 | 88 | 12.1 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1210-02 | 0.25grams | .25grams | N/A | N/A | N/A | N/A | |||
| 10-1210-05 | 0.5grams | .5grams | N/A | N/A | N/A | N/A | |||
06/02/2009 | http://www.glenres.com/ProductFiles/10-1210.html