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Catalog Number: 10-1080-xx
Description: 5-Br-dC-CE Phosphoramidite
| 5'-Dimethoxytrityl-N-benzoyl-5-bromo-2'-deoxyCytidine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C46H51BrN5O8P | M.W.: 912.82 | F.W.: 368.08 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Mild deprotection: Ammonium Hydroxide for 24 hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1080 | 5-Br-dC | 287 | 6 | 3.1 |
Frequently Asked Technical Question
QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?
RESPONSE:See: Table of Extinction Coefficients
REFERENCE(S):
B. Froehler, Gilead
M. Powell
R. Somers
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1080-02 | 0.25grams | .25grams | 2.74 | 78 | 46.8 | 29.25 | 21.27 | 15.6 | 3.9 |
| 10-1080-90 | 100µmoles | .091grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1080-02 | 0.25grams | .25grams | 4.09 | .07 | 75.4 | 47.13 | 34.27 | 4.71 | |
| 10-1080-90 | 100µmoles | .091grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1080-02 | 0.25grams | .25grams | 4.09 | .07 | 77 | 48.13 | 35 | ||
| 10-1080-90 | 100µmoles | .091grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1080.html