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Catalog Number: 10-1065-xx
Description: dmf-5-Me-isodC-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-methyl-N-dimethylformamidine-2'-deoxyisoCytidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H55N6O7P | M.W.: 798.91 | F.W.: 303.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Note: Oligos containing 5-Me-iso-dC should be stored buffered at pH 7.5 to 8.0 to avoid decomposition. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 2-3 days |
| Please Note: These products are supplied under license from EraGen Biosciences, Inc. US Patents 5,432,272, 6,001,983, 6,037,120, and 6,140,496. |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1065 | 5-Me-isodC | 260 | 6.3 |
Literature Highlights
| Glen Report 20.1: Technical Brief - New application of 5-Me-iso-dC and iso-dG |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1065-02 | 0.25grams | .25grams | 3.13 | 91 | 54.6 | 34.13 | 24.82 | 18.2 | 4.55 |
| 10-1065-90 | 100µmoles | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1065-02 | 0.25grams | .25grams | 4.67 | .067 | 87 | 54.38 | 39.55 | 5.44 | |
| 10-1065-90 | 100µmoles | .08grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1065-02 | 0.25grams | .25grams | 4.67 | .067 | 88.6 | 55.38 | 40.27 | ||
| 10-1065-90 | 100µmoles | .08grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1065.html