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Catalog Number: 10-1059-xx
Description: EDTA-C2-dT-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl- 3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite | ||
| Formula: C60H81N8O16P | M.W.: 1201.32 | F.W.: 676.53 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended. |
| Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.Note: NaOH is not compatible with dmf protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.2: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 2.08 | 56 | 33.6 | 21 | 15.27 | 11.2 | 2.8 |
| 10-1059-90 | 100µmoles | .12grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1059-95 | 50µmoles | .06grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 55.8 | 34.88 | 25.36 | 3.49 | |
| 10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1059-95 | 50µmoles | .06grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 57.4 | 35.88 | 26.09 | ||
| 10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1059-95 | 50µmoles | .06grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
11/28/2011 | http://www.glenres.com/ProductFiles/10-1059.html