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Catalog Number: 10-1058-xx
Description: Dabcyl-dT
| 5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl- 3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C63H76N9O10P | M.W.: 1150.32 | F.W.: 709.7 |
Diluent: 10% THF in Anhydrous Acetonitrile |
| Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). A 6 minute coupling time is recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1058 | Dabcyl-dT | 476 nm | 32.0 (L/mmole-cm) | 29.1 (L/mmole-cm) |
Literature Highlights
| Glen Report 11.1: NEW FLUORESCENT REAGENTS - TAMRA-dT, DABCYL-dT |
| Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 2.17 | 59 | 35.4 | 22.13 | 16.09 | 11.8 | 2.95 |
| 10-1058-90 | 100µmoles | .115grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1058-95 | 50µmoles | .058grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 3.24 | .07 | 58.4 | 36.5 | 26.55 | 3.65 | |
| 10-1058-90 | 100µmoles | .115grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1058-95 | 50µmoles | .058grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1058-02 | 0.25grams | .25grams | 3.24 | .07 | 60 | 37.5 | 27.27 | ||
| 10-1058-90 | 100µmoles | .115grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1058-95 | 50µmoles | .058grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1058.html