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Catalog Number: 10-1053-xx
Description: 5-OH-dU-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-O-Acetyl-2'-deoxyUridine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C41H49N4O10P | M.W.: 788.83 | F.W.: 306.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA:10-1601-xx,dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature or 2 hours at room temperature in Ammonium Hydroxide. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1053 | 5-OH-dU | 280 | 7.8 | 4.9 |
Literature Highlights
| Glen Report 11.1: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1053-02 | 0.25grams | .25grams | 3.17 | 92.33 | 55.4 | 34.63 | 25.18 | 18.47 | 4.62 |
| 10-1053-90 | 100µmoles | .079grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1053-02 | 0.25grams | .25grams | 4.73 | .07 | 88.2 | 55.13 | 40.09 | 5.51 | |
| 10-1053-90 | 100µmoles | .079grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1053-02 | 0.25grams | .25grams | 4.73 | .07 | 89.8 | 56.13 | 40.82 | ||
| 10-1053-90 | 100µmoles | .079grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1053.html