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Catalog Number: 10-1017-xx
Description: Pyrrolo-dC-CE Phosphoramidite
| 5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]- 2'-deoxyribonucleoside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H50N5O7P | M.W.: 767.85 | F.W.: 327.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation. |
| Deprotection: Ammonium Hydroxide for 24 hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280. |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1017 | Pyrrolo-dC | 339 | 2.36 | 229 | 17.5 | 2.41 |
Literature Highlights
| Glen Report 15.1: Pyrrolo-C - a novel fluorescent nucleoside |
| Glen Report 16.1: Minor Base and Related Novel Phosphoramidites |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 3.26 | 95.33 | 57.2 | 35.75 | 26 | 19.07 | 4.77 |
| 10-1017-90 | 100µmoles | .077grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1017-95 | 50µmoles | .038grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 4.86 | .07 | 90.8 | 56.75 | 41.27 | 5.68 | |
| 10-1017-90 | 100µmoles | .077grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1017-95 | 50µmoles | .038grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 4.86 | .07 | 92.4 | 57.75 | 42 | ||
| 10-1017-90 | 100µmoles | .077grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1017-95 | 50µmoles | .038grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
01/18/2013 | http://www.glenres.com/ProductFiles/10-1017.html