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Catalog Number: 10-1007-xx
Description: 8-Br-dA-CE Phosphoramidite
| 5'-Dimethoxytrityl-N6-dimethylaminomethylidene-8-bromo-2'-deoxyAdenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H52N8O6PBr | M.W.: 887.81 | F.W.: 392.11 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Mild Deprotection: Ammonium hydroxide for 24hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1007 | 8-Br-dA | 266 | 16.4 | 14.8 |
Literature Highlights
| Glen Report 7.1: NEW MINOR BASES |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1007-02 | 0.25grams | .25grams | 2.82 | 80.67 | 48.4 | 30.25 | 22 | 16.13 | 4.03 |
| 10-1007-90 | 100µmoles | .089grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1007-02 | 0.25grams | .25grams | 4.2 | .07 | 77.6 | 48.5 | 35.27 | 4.85 | |
| 10-1007-90 | 100µmoles | .089grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1007-02 | 0.25grams | .25grams | 4.2 | .07 | 79.2 | 49.5 | 36 | ||
| 10-1007-90 | 100µmoles | .089grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1007.html