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DEOXYURIDINE DERIVATIVES FOR INTERNAL MODIFICATION

Amino-Modifier C6 dT1 and its corresponding biotinylated product Biotin-dT have proved to be optimal for amino-modifying and biotinylating oligonucleotides within the sequence. The resulting labelled oligonucleotide has standard hybridization characteristics2 and is ideal for attaching large molecules like alkaline phosphatase3,4.

To this useful line of dU derivatives, we have added Amino-Modifier C2 dT and Carboxy-dT. In contrast to Amino-Modifier C6 dT where the label is designed to be placed where it cannot interact with the double stranded oligonucleotide, the C2 version is designed for the attachment of molecules like EDTA or alkylating reagents which can cut the complementary strand or double strand. The methyl ester of Carboxy-dT is hydrolyzed during standard deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester.

References:

(1) J.L. Ruth, C. Morgan, and A. Pasko, DNA, 1985, 4, 93.
(2) J. Telser, K.A. Cruickshank, L.E. Morrison, and T.L. Netzel, J. Am. Chem. Soc., 1989, 111, 6966-6976.
(3) E. Jablonski, E.W. Moomaw, R.H. Tullis, and J.L. Ruth, Nucleic Acids Res., 1986, 14, 6115.
(4) J.G. Farmar and M. Castaneda, Biotechniques, 1991, 11, 588-589.

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