TOM-Protected RNA Phosphoramidites
RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl
(TOM) group (TOM-Protecting-Group™) is characterized by very high
coupling efficiency along with fast, simple deprotection. High coupling
efficiency is achieved because the TOM-Protecting-Group exhibits lower
steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group
used in our alternative RNA monomers. Fast and reliable deprotection is
achieved using methylamine in ethanol/water at room temperature. A further
feature of the TOM-Protecting-Group is that during basic steps it can
not undergo 2’ to 3’ migration. This migration under basic
conditions leads to non-biologically active 2’-5’ linkages
when using the TBDMS group. These features allow the TOM-Protected monomers
to produce longer oligonucleotides. TOM-Protected RNA monomers are also
fully compatible with minor bases with 2’-O-TBDMS protection.
| 10-3004-02 |
0.25g |
75.00 |
| |
10-3004-05 |
0.5g |
150.00 |
| |
10-3004-10 |
1.0g |
275.00 |
| 10-3014-02 |
0.25g |
75.00 |
| |
10-3014-05 |
0.5g |
150.00 |
| |
10-3014-10 |
1.0g |
275.00 |
| 10-3024-02 |
0.25g |
75.00 |
| |
10-3024-05 |
0.5g |
150.00 |
| |
10-3024-10 |
1.0g |
275.00 |
| 10-3034-02 |
0.25g |
75.00 |
| |
10-3034-05 |
0.5g |
150.00 |
| |
10-3034-10 |
1.0g |
275.00 |
|
INTELLECTUAL PROPERTY
TOM-Protecting-Group™
TOM-RNA Phosphoramidites are supplied under agreement
with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered
by US Patent No. 5,986,084.
TOM-Protecting-Group is a trademark of QIAGEN
|
TBDMS-Protected rna phosphoramidites
Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).
| 10-3003-02 |
0.25g |
40.00 |
| |
10-3003-05 |
0.5g |
80.00 |
| |
10-3003-10 |
1.0g |
160.00 |
| 10-3015-02 |
0.25g |
40.00 |
| |
10-3015-05 |
0.5g |
80.00 |
| |
10-3015-10 |
1.0g |
160.00 |
| 10-3025-02 |
0.25g |
40.00 |
| |
10-3025-05 |
0.5g |
80.00 |
| |
10-3025-10 |
1.0g |
160.00 |
| 10-3030-02 |
0.25g |
40.00 |
| |
10-3030-05 |
0.5g |
80.00 |
| |
10-3030-10 |
1.0g |
160.00 |
HT RNA Phosphoramidites
| 10-3003-2HT |
2.0g |
|
| 10-3015-2HT |
2.0g |
|
| 10-3025-2HT |
2.0g |
|
| 10-3030-2HT |
2.0g |
|
LC RNA Phosphoramidites
| 10-3003-10 |
1.0g |
|
| 10-3015-10 |
1.0g |
|
| 10-3025-10 |
1.0g |
|
| 10-3030-10 |
1.0g |
|
UltraMild TBDMS rna Phosphoramidites
| 10-3000-02 |
0.25g |
75.00 |
| |
10-3000-05 |
0.5g |
150.00 |
| |
10-3000-10 |
1.0g |
275.00 |
| 10-3015-02 |
0.25g |
40.00 |
| |
10-3015-05 |
0.5g |
80.00 |
| |
10-3015-10 |
1.0g |
160.00 |
| 10-3021-02 |
0.25g |
75.00 |
| |
10-3021-05 |
0.5g |
150.00 |
| |
10-3021-10 |
1.0g |
275.00 |
| 10-3030-02 |
0.25g |
40.00 |
| |
10-3030-05 |
0.5g |
80.00 |
| |
10-3030-10 |
1.0g |
160.00 |
|
OTHER INSTRUMENT
TYPES
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Expedite |
E |
Beckman Oligo 1000 |
B |
Pharmacia Gene Assembler |
P |
Mermade |
M |
Applied Biosystems 3900 |
A |
Expedite |
E |
Mermade |
M |
|
Please inquire for availability
of columns for other
instrument types.
|
HT RNA
Minimum order: 40g total
Products may be combined to 40g total. Please request a quote.
LC RNA
Minimum order: 20g total
Products may be combined to 20g total. Please request a quote.
ABBREVIATIONS
Bz = Benzoyl
CNEt = Cyanoethyl
CPG = Controlled Pore Glass
dmf = Dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
iPr = Isopropyl
lcaa = long chain alkylamino
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TBDMS = t-Butyl-dimethylsilyl
|
TBDMS rna supports
ABI-style columns are supplied for 1 µmole and 0.2
µmole scales unless otherwise requested (see note box).
| 20-3300-01 |
0.1g |
40.00 |
| |
20-3300-02 |
0.25g |
95.00 |
| |
20-3300-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3400-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3400-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3400-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3400-14 |
Pack of 1 |
300.00 |
| 20-3303-01 |
0.1g |
40.00 |
| |
20-3303-02 |
0.25g |
95.00 |
| |
20-3303-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3403-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3403-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3403-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3403-14 |
Pack of 1 |
300.00 |
| 20-3315-01 |
0.1g |
40.00 |
| |
20-3315-02 |
0.25g |
95.00 |
| |
20-3315-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3415-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3415-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3415-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3415-14 |
Pack of 1 |
300.00 |
| 20-3321-01 |
0.1g |
40.00 |
| |
20-3321-02 |
0.25g |
95.00 |
| |
20-3321-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3421-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3421-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3421-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3421-14 |
Pack of 1 |
300.00 |
| 20-3329-01 |
0.1g |
40.00 |
| |
20-3329-02 |
0.25g |
95.00 |
| |
20-3329-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3429-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3429-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3429-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3429-14 |
Pack of 1 |
300.00 |
| 20-3324-01 |
0.1g |
40.00 |
| |
20-3324-02 |
0.25g |
95.00 |
| |
20-3324-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3424-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3424-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3424-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3424-14 |
Pack of 1 |
300.00 |
| 20-3330-01 |
0.1g |
40.00 |
| |
20-3330-02 |
0.25g |
95.00 |
| |
20-3330-10 |
1.0g |
355.00 |
| 1 µmole columns |
20-3430-41 |
Pack of 4 |
100.00 |
| 0.2 µmole columns |
20-3430-42 |
Pack of 4 |
75.00 |
| 10 µmole column (ABI) |
20-3430-13 |
Pack of 1 |
225.00 |
| 15 µmole column (Expedite) |
20-3430-14 |
Pack of 1 |
300.00 |
|
OTHER INSTRUMENT TYPES
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Expedite |
E |
Beckman Oligo 1000 |
B |
Pharmacia Gene Assembler |
P |
Mermade |
M |
Applied Biosystems 3900 |
A |
Expedite |
E |
Mermade |
M |
|
Please inquire for availability
of columns for other
instrument types.
|
|
