ALTERNATIVE SOLVENTS/REAGENTS

Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution. < < <

Item	Catalog No.	Pack	Price ($)
Activator
5-Ethylthio-1H-tetrazole (ETT)	30-3040-10	1g	35.00
(Dissolve 1g in 31mL anhydrous acetonitrile	30-3040-20	2g	60.00
for a 0.25M solution)	30-3040-25	25g	500.00
0.25M 5-Ethylthio-1H-tetrazole in Acetonitrile
(Applied Biosystems)	30-3140-45	45mL	40.00
	30-3140-52	200mL	100.00
	30-3140-57	450mL	200.00
	30-3140-62	2L	760.00
	30-3142-52	200mL	100.00
	30-3140-57	450mL	200.00
4,5-Dicyanoimidazole (DCI), crystalline	30-3050-10	1g	35.00
(Dissolve 1g in 34mL anhydrous acetonitrile	30-3050-25	25g	500.00
for a 0.25M solution)
A license may be required from Isis Pharmaceuticals, Inc. to use DCI as an activator in oligonucleotide synthesis as claimed in U.S. Patent Numbers 5,734,041; 6,031,092; 6,160,109 and foreign equivalents.
0.25M DCI in Acetonitrile
(Applied Biosystems)	30-3150-45	45mL	40.00
	30-3150-52	200mL	100.00
	30-3150-57	450mL	200.00
	30-3150-62	2L	760.00
(Expedite)	30-3152-52	200mL	100.00
	30-3150-57	450mL	200.00
5-Benzylthio-1H-tetrazole	30-3070-10	1g	35.00
(Dissolve 1g in 21.3mL anhydrous acetonitrile	30-3070-20	2g	60.00
for a 0.25M solution)	30-3070-25	25g	500.00
5-Benzylthio-1H-tetrazole in Acetonitrile
(Applied Biosystems)	30-3170-45	45mL	40.00
	30-3170-52	200mL	100.00
	30-3170-57	450mL	200.00
	30-3170-62	2L	760.00
(Expedite)	30-3172-52	200mL	100.00
	30-3170-57	450mL	200.00
Cap Mix A
THF/Lutidine/Ac2O	40-4010-52	200mL	30.00
	40-4010-57	450mL	72.00
	40-4010-62	2L	325.00
THF/Ac2O (9:1)	40-4012-62	2L	275.00
Cap Mix B
6.5% DMAP in THF (Cap B solutions containing DMAP are preferred by some researchers for preparing long oligos.)	40-4020-52	200mL	42.00
10% MeIm in THF (This Cap B solution is identical to the formulation produced by Applied Biosystems.)	40-4120-52	200mL	30.00
	40-4120-57	450mL	72.00
	40-4120-62	2L	325.00
10% MeIm in THF/Pyridine (8:1)	40-4122-62	2L	325.00
Oxidizing Solution
0.02M I2 in THF/Pyridine/H2O	40-4132-62	2L	325.00
Deblocking Mix
3% DCA/DCM (DCA solutions are more mildly acidic than the TCA equivalents, possibly causing less depurination of dA sites.)	40-4040-57	450mL	36.00
	40-4040-62	2L	144.00
2.5% DCA/DCM	40-4042-57	450mL	36.00
	40-4042-62	2L	144.00
DCA	40-4044-54	120mL	60.00
(Add to 4L of DCM to make a 3% solution.)

Alternative Capping Reagent

The major impurities generated during oligonucleotide synthesis are n-1 and, to a lesser extent, n-2 deletion sequences. Failure to cap, and the resulting generation of excess n-1 deletion sequences, present particular problems in trityl-on purifications and in the synthesis of long oligonucleotides for gene construction and cloning. One option is to use the phosphoramidite of diethylene glycol monoethyl ether, UniCap, as the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.

Item	Catalog No.	Pack	Price ($)
UniCap
UniCap Phosphoramidite	10-4410-02	0.25g	50.00
	10-4410-05	0.5g	100.00
	10-4410-10	1.0g	200.00
	10-4410-20	2.0g	400.00