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purification

POLY-PAK™ PURIFICATION

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is delivered with cartridges, barrels, and packing and is available on request.

ItemCatalog No. PackPrice ($)
Packing, Cartridges and Barrels
Poly-Pak™ Packing 60-1000-05 5g 70.00
  60-1000-25 25g 350.00*
Poly-Pak™ Cartridge 60-1100-01 each 8.00
  60-1100-10 Pack of 10 80.00
Poly-Pak™ Barrel 60-2100-30 Pack of 30 DISCONTINUED
Poly-Pak™ II Cartridge 60-3100-01 each 12.00
  60-3100-10 Pack of 10 120.00
Poly-Pak™ II Barrel 60-4100-30 Pack of 30 DISCONTINUED
*Price change 1/15/2010
Reagents
Wash Solvent Kit 60-4900-01 each DISCONTINUED
(Sufficient for use with up to 10 Poly-Pak cartridges)
2.0M Triethylamine Acetate (TEAA) 60-4110-52 200mL 60.00
HPLC Grade 60-4110-57 450mL 120.00
  60-4110-60 960mL 200.00
  60-4110-62 2L 400.00
2% Aqueous Trifluoroacetic Acid 60-4040-57 450mL 36.00
3% Aqueous Trifluoroacetic Acid 60-4041-57 450mL 36.00
4% Aqueous Trifluoroacetic Acid 60-4042-57 450mL 36.00

polypak

Poly-Pak Cartridge Used Manually

 

 

 

 

 

 

 

 

Poly-Pak™ is a trademark of Glen Research Corporation

Glen-Pak™ Cartridges

Glen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5’ terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses.

Please see our USER GUIDE TO GLEN-PAK™ PURIFICATION

ItemCatalog No. PackPrice ($)
DNA Purification

Cartridges
Glen- Pak™DNA Purification Cartridge 60-5100-10

 Pack of 10

 80.00
(For use in vacuum manifolds

 60-5100-30

 Pack of 30

 200.00
and high-throughput devices)

 60-5100-96

 Pack of 96

 475.00
Glen-Pak™ DNA Purification Cartridge

 60-5200-01

 each

 8.00
(For use with disposable syringes)

 60-5200-10

 Pack of 10

 80.00
Glen-Pak™ Cartridge 3g

 60-5300-01

 Pack of 1

 150.00
Glen-Pak™ DNA 30mg 96-Well Plate

 60-5400-01

 Pack of 1

 475.00
RNA Purification

Cartridges
Glen-Pak™ RNA Purification Cartridge

 60-6100-10

 Pack of 10

 95.00
(For use in vacuum manifolds

 60-6100-30

 Pack of 30

 225.00
and high-throughput devices)

 60-6100-96

 Pack of 96

 575.00
Glen-Pak™ RNA Purification Cartridge

 60-6200-01

 each

 9.50
(For use with disposable syringes)

 60-6200-10

 Pack of 10

 95.00
Reagents
RNA Quenching Buffer

 60-4120-82

 250mL

 80.00
60-4120-80

 1L

 200.00
Racks and Seals
Adapter Rack
(for 96 well manifolds)		 60-0010-01

 each

 10.00
Seal for Adaptor Rack
(for unused wells on Adapter Rack)		 60-0020-01

 each

 20.00
glenpaksSee Glen Report 19.2

 

See Also

Poly-Pak Reagents p116

Fluoro-Pak™ purification

The fluorous affinity purification of oligonucleotides is a quick and simple affinity-based method for the purification of oligonucleotides that relies on the strong interaction of fluorous-tagged oligonucleotides (made with fluorous-tagged phosphoramidites) with the fluorinated adsorbent present in Fluoro-Pak™ columns. Fluorous affinity purification is operationally similar to DMT-on purification using a reverse-phase (RP) adsorbent, e.g., RP cartridge purification, except that it involves a much stronger affinity interaction and is thus able to afford higher selectivities and recoveries, even with long oligos.

 

Item Catalog No. Pack Price ($)
F-DMT-dA-CE Phosphoramidite 10-1400-90 100 µmole 75.00
  10-1400-02 0.25g 140.00
F-DMT-dC-CE Phosphoramidite 10-1410-90 100 µmole 75.00
  10-1410-02 0.25g 140.00
F-DMT-dG-CE Phosphoramidite 10-1420-90 100 µmole 75.00
  10-1420-02 0.25g 140.00
F-DMT-dT-CE Phosphoramidite 10-1430-90 100 µmole 75.00
  10-1430-02 0.25g 140.00
Fluorous Chemical Phosphorylation Reagent II 10-1904-90 100 µmole 95.00
  10-1904-02 0.25g 195.00
Columns
Fluoro-Pak™columns (75 mg adsorbent for ≤200 nmol syntheses) 61-2100-10 Pack of 10 100.00
(with Luer adaptor) 61-2100-10A Pack of 10 100.00
 
Fluoro-Pak™ II columns (150 mg adsorbent, for ≤1µmol syntheses) 61-4100-10 Pack of 10 135.00
(with Luer adaptor) 61-4100-10A Pack of 10 135.00
 
Loading Buffer 61-4120-52 200 mL 35.00

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers
For Instrument type
Add
Expedite
E
Beckman Oligo 1000
B
Pharmacia Gene Assembler
P
Mermade
M

Columns
For Instrument type
Add
Applied Biosystems 3900
A
Expedite
E
Mermade
M
Please inquire for availability
of columns for other
instrument types.

INTELLECTUAL PROPERTY

“Fluoro-Pak” is a trademark of Berry & Associates, Inc. Products for Fluorous Affinity Purification of Oligonucleotides: Patents applied for, Berry & Associates, Inc. Further, the use of these products is licensed under U.S. Patents 6,673,539, 6,156,896; 5,859,247; and 5,777,121 and one or more pending patents owned or controlled by Fluorous Technologies, Inc.

See Also

Poly-Pak Reagents p116

Oligo-Affinity Support

Oligo-affinity supports (OAS) should ideally be compatible with automated synthesis, should be non-friable, should not shrink or swell, and should have low non-specific binding of the proteins or DNA. On the support shown below is an Adenosine residue attached through the exocyclic amino group. In this way, synthesis progresses regularly on removal of the 5’-DMT group. However, on treatment with ammonium hydroxide, the oligo is not cleaved from the support. This matrix can then be used as an affinity support for a complementary segment of DNA or RNA. Alternatively, the complementary strand can be annealed to the support and the double stranded DNA can be used as an affinity support for purifying DNA binding proteins.

We expect that OAS PS will be used for purification of components from biological fluids.

Item Catalog No. Pack Price ($)
Oligo-Affinity Support (PS) 26-4001-01 0.1g 180.00
(OAS PS) 26-4001-02 0.25g 425.00
  26-4001-10 1.0g 1590.00
Oligo-Affinity Support (PS)
1 µmole TWIST columns 26-4101-41 Pack of 4 300.00

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers
For Instrument type
Add
Expedite
E
Beckman Oligo 1000
B
Pharmacia Gene Assembler
P
Mermade
M

Columns
For Instrument type
Add
Applied Biosystems 3900
A
Expedite
E
Mermade
M
Please inquire for availability
of columns for other
instrument types.

http://www.glenres.com/Catalog/purification.html

  
Please contact Glen Research if you have any questions or comments!
 
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