STERLING CE PHOSPHORAMIDITES

The PolyPlex DNA synthesizer is a 96 well parallel array synthesizer developed by GeneMachines, now a division of Genomic Solutions (http://www.genomicsolutions.com/). Phosphoramidite monomers are packaged in 60mL amber vials (2g) or 240mL amber bottles (10g) for dissolving at a concentration of 1g/20mL and are connected directly to the instruments.

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. To avoid potential crystallization of tetrazole from the 0.45M tetrazole activator solution, parallel synthesizers typically use 5-ethylthio-1H-tetrazole (ETT) or 4,5-dicyanoimidazole (DCI) as activator. Both are used at a concentration of 0.25M in acetonitrile, which is far below the level at which crystallization may occur.

Regular 500Å or 1000Å supports may be used to fill the wells. However, this requires each plate to be prepared with each nucleoside accurately in all wells. A universal support clearly removes the need for four specific supports and makes preparing plates straightforward.