The physical data table contains information which is unique to
each monomer phosphoramidite. The molecular weight (MW) is the formula weight
of the fully-protected monomer phosphoramidite. The MW is used to calculate
the volume of solvent required to dilute 0.25g of the monomer to give a final
0.1M concentration. This figure is also shown in the table. The unit molecular
weight (Unit FW) is the formula weight of each monomer once inserted into an
oligonucleotide with all protecting groups removed. To obtain the molecular
weight of a specific oligonucleotide, the following formula is used:
10-1430 |
F-DMT-dT-CE Phosphoramidite |
1,190.93 |
304.20 |
0.25g/2.10mL |
10-1501 |
1-Me-dA-CE Phosphoramidite |
814.31 |
328.24 |
0.25g/3.07mL |
10-1511 |
5,6-dihydro-5-aza-dC-CE Phosphoramidite |
787.89 |
292.18 |
0.25g/3.17mL |
10-1520 |
8-D-dmf-dG-CE Phosphoramidite |
825.91 |
330.21 |
0.25g/3.03mL |
10-1530 |
5,6-Dihydro-dT-CE Phosphoramidite |
746.84 |
306.21 |
0.25g/3.35mL |
10-1531 |
N3-Cyanoethyl-dT |
797.88 |
357.26 |
0.25g/3.13mL |
10-1532 |
5’-Dabsyl-dT |
729.78 |
591.53 |
0.25g/3.43mL |
10-1535 |
NHS-Carboxy-dT |
897.91 |
varies, -CO2H=360.22 |
0.25g/2.78mL |
10-1536 |
Fmoc Amino-Modifier C6 dT |
1,121.28 |
varies, -NH2=458.41 |
0.25g/2.23mL |
10-1550 |
5,6-Dihydro-dU-CE Phosphoramidite |
732.81 |
292.19 |
0.25g/3.41mL |
10-1590 |
Pyrene-dU |
955.04 |
514.42 |
0.25g/2.62mL |
10-1591 |
Perylene-dU |
1,005.10 |
564.48 |
0.25g/2.49mL |
10-1601 |
Pac-dA-CE Phosphoramidite |
887.97 |
313.21 |
0.25g/2.82mL |
10-1621 |
iPr-Pac-dG-CE Phosphoramidite |
946.05 |
329.21 |
0.25g/2.64mL |
10-1900 |
Chemical Phosphorylation Reagent |
656.77 |
79.98 |
0.25g/3.81mL |
10-1901 |
Chemical Phosphorylation Reagent II |
722.82 |
79.98 |
0.25g/3.46mL |
10-1902 |
Solid Chemical Phosphorylation Reagent II |
692.78 |
79.98 |
0.25g/3.61mL |
10-1904 |
Fluorous Chemical Phosphorylation Reagent II |
1,168.92 |
79.98 |
0.25g/2.14mL |
10-1905 |
5’-Amino-Modifier 5 |
577.71 |
167.10 |
0.25g/4.33mL |
10-1906 |
5’-Amino-Modifier C6 |
589.76 |
179.16 |
0.25g/4.24mL |
10-1907 |
5’-DMS(O)MT-Amino-Modifier C6 |
681.34 |
179.16 |
0.25g/3.67mL |
10-1908 |
5’-Hexynyl Phosphoramidite |
298.36 |
160.11 |
0.25g/8.38mL |
10-1909 |
Spacer Phosphoramidite 9 |
652.77 |
212.14 |
0.25g/3.83mL |
10-1912 |
5’-Amino-Modifier C12 |
673.92 |
263.32 |
0.25g/3.71mL |
10-1913 |
Spacer Phosphoramidite C3 |
578.69 |
138.06 |
0.25g/4.32mL |
10-1914 |
dSpacer CE Phosphoramidite |
620.73 |
180.10 |
0.25g/4.03mL |
10-1915 |
Pyrrolidine CE Phosphoramidite |
841.97 |
194.15 |
0.25g/2.97mL |
10-1916 |
5’-Amino-Modifier C6-TFA |
413.42 |
179.16 |
0.25g/6.05mL |
10-1918 |
Spacer Phosphoramidite 18 |
784.93 |
344.30 |
0.25g/3.18mL |
10-1920 |
Symmetric Doubler Phosphoramidite |
1,095.32 |
351.31 |
0.25g/2.28mL |
10-1921 |
Asymmetric Doubler Phosphoramidite |
1,001.17 |
351.31 |
0.25g/2.50mL |
10-1922 |
Trebler Phosphoramidite |
1,417.72 |
370.33 |
0.25g/1.76mL |
10-1923 |
5’-Amino-Modifier C3-TFA |
371.34 |
137.08 |
0.25g/6.73mL |
10-1924 |
Abasic Phosphoramidite |
1,011.55 |
196.09 |
0.25g/2.47mL |
10-1925 |
Long Trebler Phosphoramidite |
1,475.78 |
428.41 |
0.25g/1.69mL |
10-1926 |
5’-Thiol-Modifier C6 |
576.78 |
196.20 |
0.25g/4.33mL |
10-1928 |
Spacer C12 CE Phosphoramidite |
704.93 |
264.30 |
0.25g/3.55mL |
10-1932 |
5’-Amino-dT-CE Phosphoramidite |
713.81 |
303.21 |
0.25g/3.50mL |
10-1933 |
5’-Aldehyde-Modifier C2 Phosphoramidite |
480.58 |
245.14 |
0.25g/5.20mL |
10-1934 |
5-Formylindole-CE Phosphoramidite |
763.86 |
323.24 |
0.25g/3.27mL |
10-1935 |
5’-Carboxy-Modifier C10 |
485.56 |
varies, -CO2H=250.23 |
0.25g/5.15mL |
10-1936 |
Thiol-Modifier C6 S-S |
769.05 |
196.20 |
0.25g/3.25mL |
10-1937 |
Dithiol Phosphoramidite (DTPA) |
654.81 |
disulfide=214.19 |
0.25g/3.82mL |
|
|
|
dithiol=216.20 |
|
10-1952 |
DesthiobiotinTEG Phosphoramidite |
980.19 |
539.56 |
0.25g/2.55mL |
10-1953 |
Biotin Phosphoramidite |
876.10 |
435.48 |
0.25g/2.85mL |
10-1955 |
BiotinTEG Phosphoramidite |
1,010.24 |
569.61 |
0.25g/2.47mL |
10-1963 |
Fluorescein Phosphoramidite |
1,207.50 |
598.56 |
0.25g/2.07mL |
10-1964 |
6-Fluorescein Phosphoramidite |
1,176.35 |
566.48 |
0.25g/2.13mL |
10-1973 |
Acridine Phosphoramidite |
891.53 |
450.86 |
0.25g/2.80mL |
10-1975 |
Cholesteryl-TEG Phosphoramidite |
1,196.60 |
755.97 |
0.25g/2.09mL |
10-1982 |
Psoralen C2 Phosphoramidite |
502.55 |
364.29 |
0.25g/4.97mL |