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GE Healthcare Life Sciences Instruments

STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination.

Item Catalog No. Pack Price ($)
Gene Assembler
dA-CE Phosphoramidite 10-1000-P4 0.4g 20.00
  10-1000-1P 1.0g 50.00
dC-CE Phosphoramidite 10-1010-P4 0.4g 20.00
  10-1010-1P 1.0g 50.00
Ac-dC-CE Phosphoramidite 10-1015-P4 0.4g 20.00
  10-1015-1P 1.0g 50.00
dG-CE Phosphoramidite 10-1020-P4 0.4g 20.00
  10-1020-1P 1.0g 50.00
dmf-dG-CE Phosphoramidite 10-1029-P4 0.4g 20.00
  10-1029-1P 1.0g 50.00
dT-CE Phosphoramidite 10-1030-P4 0.4g 20.00
  10-1030-1P 1.0g 50.00
Äkta Oligopilot
dA-CE Phosphoramidite 10-1000-20 2.0g 100.00
  10-1000-50 5.0g 250.00
dC-CE Phosphoramidite 10-1010-20 2.0g 100.00
  10-1010-50 5.0g 250.00
Ac-dC-CE Phosphoramidite 10-1015-20 2.0g 100.00
  10-1015-50 5.0g 250.00
dG-CE Phosphoramidite 10-1020-25 2.0g 100.00
  10-1020-50 5.0g 250.00
dmf-dG-CE Phosphoramidite 10-1029-20 2.0g 100.00
  10-1029-50 5.0g 250.00
dT-CE Phosphoramidite 10-1030-20 2.0g 100.00
10-1030-50 5.0g 250.00

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

 

See Also

HT DNA Phosphoramidites p13

STERLING SOLVENTS/REAGENTS

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination.

Item Catalog No. Pack Price ($)
Diluent
Acetonitrile, anhydrous 40-4050-45 45mL 12.00
  40-4050-50 100mL 16.00
Äkta Oligopilot
Activator
0.40M Tetrazole in Acetonitrile 30-3105-71 1L 380.00
Cap Mix A
Acetonitrile/MeIm 40-4015-71 1L 145.00
Cap Mix B1*
Acetonitrile/Ac2O 40-4026-75 500mL *
Cap Mix B2*
Acetonitrile/Lutidine 40-4027-75 500mL *
CapB1 + CapB2
  (*Cap Mix B1 and Cap Mix B2 are combined immediately before use and must be ordered together.)
160.00
Oxidizing Solution
0.05M I2 in Pyridine/H2O 40-4035-71 1L 160.00
Deblocking Mix
3% DCA/DCM 40-4040-71 1L 80.00
3% TCA/DCM 40-4140-71 1L 80.00
3% DCA/Toluene 40-4240-71 1L 145.00

ABBREVIATIONS


Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloroacetic Acid
DCM = Dichloromethane
I2 = Iodine
MeIm = 1-Methylimidazole
µm = micromole(s)

 

See Also

Alternative Activators p13

 

*Cap Mix B1 and Cap Mix B2 are combined immediately before use and must be ordered together.

STERLING SUPPORTS

All Glen Research supports use the standard long chain alkylamino (lcaa) linker and are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis.

Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
dA dC dG dT Ac-dC    
500Å Columns
20-2500-P2 20-2510-P2 20-2520-P2 20-2530-P2   10x0.2µm 120.00
20-2500-P1 20-2510-P1 20-2520-P1 20-2530-P1   10x1.3µm 120.00
1000Å Columns
        20-2515-P2 10x0.2µm 120.00
        20-2515-P1 10x1.3µm 120.00
Polystyrene Support
27-5110-P2 10x0.2µm 100.00
        27-5110-P1 10x1.3µm 150.00

 

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Please contact Glen Research if you have any questions or comments!
 
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