STERLING CE PHOSPHORAMIDITES

MerMade synthesizers belong to a family of synthesizers, including the column-based MerMade 4, MerMade 6, and MerMade 12 instruments and the parallel array synthesizers, MerMade 192 and MerMade 384, manufactured by BioAutomation Corporation in Plano, TX. Their web site can be found at: http://www.BioAutomation.com. Phosphoramidite monomers are packaged in 30mL and 240mL amber bottles for dissolving at a concentration of 1g/20mL and are connected directly to the instrument.

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. To avoid potential crystallization of tetrazole from the 0.45M tetrazole activator solution, parallel synthesizers typically use 5-ethylthio-1H-tetrazole (ETT) as activator. ETT is used at a concentration of 0.25M in acetonitrile, which is far below the level at which crystallization may occur.

Columns containing 1000Å CPG are available in packs of 200 to fit MerMade plates. Regular 500Å or 1000Å supports, listed on page 8, may also be used to fill the wells of regular 96 well plates. However, this requires each plate to be prepared with each nucleoside accurately in all wells. A universal support clearly removes the need for four specific supports and makes preparing plates straightforward.