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OTHER INSTRUMENT TYPESAll minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Item | Catalog No. | Pack | Price ($) |
| 3'-Dabsyl CPG | 20-5911-01 | 0.1g | 120.00 |
| 20-5911-10 | 1.0g | 975.00 | |
| 1 µmole columns | 20-5911-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-5911-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-5911-13 | Pack of 1 | 350.00 |
| 15 µmole column (Expedite) | 20-5911-14 | Pack of 1 | 000.00 |
| 3'-Dabcyl CPG | 20-5912-01 | 0.1g | 120.00 |
| 20-5912-10 | 1.0g | 975.00 | |
| 1 µmole columns | 20-5912-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-5912-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-5912-13 | Pack of 1 | 350.00 |
| 15 µmole column (Expedite) | 20-5912-14 | Pack of 1 | 000.00 |
| 3'-Dabcyl PS | |||
200 nmole
columns (ABI 3900) |
26-5912-52 | Pack of 10 | 300.00 |
| 40 nmole columns (ABI 3900) | 26-5912-55 | Pack of 10 | 300.00 |
| Dabcyl-dT | 10-1058-95 | 50 µmole | 180.00 |
| 10-1058-90 | 100 µmole | 325.00 | |
| 10-1058-02 | 0.25g | 675.00 | |
| 5'-Dabcyl Phosphoramidite | 10-5912-95 | 50 µmole | 125.00 |
| 10-5912-90 | 100 µmole | 225.00 | |
| 10-5912-02 | 0.25g | 650.00 | |
| 5'-Dabsyl-dT | 10-1532-90 | 100 µmole | 250.00 |
| 10-1532-02 | 0.25g | 775.00 | |
(1) S. Tyagi and F.R. Kramer, Nature Biotechnology, 1996, 4, 303-308.
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
Glen Research biotin phosphoramidites for direct labelling of synthetic oligonucleotides exhibit the following features:
1. All are soluble in acetonitrile at concentrations useful for DNA synthesis.
2. All include a DMT group for cartridge purifications which is essential for the preparation of biotinylated PCR primers because of the potential for cross contamination in HPLC purifications.
3. For the development of diagnostic probes, biotin phosphoramidite is capable of branching to allow multiple biotins to be introduced at the 3’- or 5’-terminus while biotin-dT can replace dT residues within the oligonucleotide sequence. BiotinTEG Phosphoramidite contains a 15 atom mixed polarity spacer arm based on a triethylene glycol. 5’-Biotin phosphoramidite can be added ONLY ONCE to the 5’-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques.
4. Protected Biotin Serinol Phosphoramidite and CPG are protected with a t-butylbenzoyl group on the biotin ring. This group is designed to stop any phosphoramidite reactions at this active position in biotin. This protection avoids branching when using nucleophilic activators like DCI. The protecting group is easily removed during oligonucleotide cleavage and deprotection. The BiotinLC versions are similarly protected and should be useful for the synthesis of highly sensitive biotinylated probes.
| Item | Catalog No. | Pack | Price ($) |
| Biotin Phosphoramidite | 10-1953-95 | 50 µmole | 165.00 |
| 10-1953-90 | 100 µmole | 295.00 | |
| 10-1953-02 | 0.25g | 675.00 | |
| BiotinTEG Phosphoramidite | 10-1955-95 | 50 µmole | 165.00 |
| 10-1955-90 | 100 µmole | 295.00 | |
| 10-1955-02 | 0.25g | 675.00 | |
| Protected Biotin Serinol Phosphoramidite | 10-1993-95 | 50 µmole | 165.00 |
| 10-1993-90 | 100 µmole | 295.00 | |
| 10-1993-02 | 0.25g | 675.00 | |
| Protected BiotinLC Serinol Phosphoramidite | 10-1995-95 | 50 µmole | 205.00 |
| 10-1995-90 | 100 µmole | 365.00 | |
| 10-1995-02 | 0.25g | 675.00 |
Biotin-dT can replace dT residues within the oligonucleotide sequence. 5’-Biotin phosphoramidite can be added ONLY ONCE to the 5’-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5’-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3’ terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.
ABI-style vials and columns are supplied unless otherwise requested (see note box).
| Item | Catalog No. | Pack | Price ($) |
| 5'-Biotin Phosphoramidite | 10-5950-95 | 50 µmole | 125.00 |
| 10-5950-90 | 100 µmole | 225.00 | |
| 10-5950-02 | 0.25g | 650.00 | |
| Biotin-dT | 10-1038-95 | 50 µmole | 180.00 |
| 10-1038-90 | 100 µmole | 325.00 | |
| 10-1038-02 | 0.25g | 675.00 | |
| PC Biotin Phosphoramidite | 10-4950-95 | 50 µmole | 135.00 |
| 10-4950-90 | 100 µmole | 250.00 | |
| 10-4950-02 | 0.25g | 675.00 | |
| DesthiobiotinTEG Phosphoramidite | 10-1952-95 | 50 µmole | 185.00 |
| 10-1952-90 | 100 µmole | 335.00 | |
| 10-1952-02 | 0.25g | 775.00 | |
| 3'-BiotinTEG-CPG | 20-2955-01 | 0.1g | 120.00 |
| 20-2955-10 | 1.0g | 995.00 | |
| 0.2 µmole columns | 20-2955-42 | Pack of 4 | 120.00 |
| 1 µmole columns | 20-2955-41 | Pack of 4 | 200.00 |
| 10 µmole column (ABI) | 20-2955-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2955-14 | Pack of 1 | 450.00 |
| 3'-BiotinTEG PS | |||
| 200 nmole columns (ABI 3900) | 26-2955-52 | Pack of 10 | 300.00 |
| 40 nmole columns (ABI 3900) | 26-2955-55 | Pack of 10 | 300.00 |
| 3’-Protected Biotin Serinol CPG | 20-2993-01 | 0.1g | 120.00 |
| 20-2993-10 | 1.0g | 995.00 | |
| 0.2 µmole columns | 20-2993-42 | Pack of 4 | 120.00 |
| 1 µmole columns | 20-2993-41 | Pack of 4 | 200.00 |
| 10 µmole column (ABI) | 20-2993-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2993-14 | Pack of 1 | 450.00 |
| 3’-Protected BiotinLC Serinol CPG | 20-2995-01 | 0.1g | 120.00 |
| 20-2995-10 | 1.0g | 995.00 | |
| 0.2 µmole columns | 20-2995-42 | Pack of 4 | 120.00 |
| 1 µmole columns | 20-2995-41 | Pack of 4 | 200.00 |
| 10 µmole column (ABI) | 20-2995-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2995-14 | Pack of 1 | 450.00 |
| DesthiobiotinTEG-CPG | 20-2952-01 | 0.1g | 140.00 |
| 20-2952-10 | 1.0g | 1150.00 | |
| 0.2 µmole columns | 20-2952-42 | Pack of 4 | 140.00 |
| 1 µmole columns | 20-2952-41 | Pack of 4 | 230.00 |
| 10 µmole column (ABI) | 20-2952-13 | Pack of 1 | 345.00 |
| 15 µmole column (Expedite) | 20-2952-14 | Pack of 1 | 020.00 |
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
5’-Biotin Phosphoramidite is supplied under license. US Patent 5,247,081 and corresponding patents worldwide.
5’-Fluorescein phosphoramidite contains no 4,4’-dimethoxytrityl (DMT) group and can be added only once at the 5’-terminus, thereby terminating synthesis. This product is prepared using the 6-carboxyfluorescein derivative. The tetrachloro-, hexachloro-and dichloro-dimethoxy-fluorescein phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers. Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling. The analogous structure, 6-Fluorescein Phosphoramidite, prepared using 6-FAM, is also available, along with 6-Fluorescein Serinol Phosphoramidite. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.
We offer five fluorescein supports. Fluorescein CPG has traditionally been used to add the fluorescein label at the 3’-terminus. The analogous structure, 3’-(6-Fluorescein) CPG, prepared using 6-FAM, is now also available, along with 6-Fluorescein Serinol CPG. We also offer 3’-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM). Both are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. 3’-(6-FAM) CPG allows effective blockage of the 3’-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generates the fluorescein labelled oligos.
The spectral characteristics of these dyes are detailed on the right side of this page.
| Item | Catalog No. | Pack | Price ($) |
| 5'-Fluorescein Phosphoramidite | 10-5901-95 | 50 µmole | 110.00 |
| (6-FAM) | 10-5901-90 | 100 µmole | 215.00 |
| 10-5901-02 | 0.25g | 075.00 | |
| 5'-Hexachloro-Fluorescein Phosphoramidite | 10-5902-95 | 50 µmole | 190.00 |
| (HEX) | 10-5902-90 | 100 µmole | 375.00 |
| 10-5902-02 | 0.25g | 875.00 | |
| 5'-Tetrachloro-Fluorescein Phosphoramidite | 10-5903-95 | 50 µmole | 180.00 |
| (TET) | 10-5903-90 | 100 µmole | 350.00 |
| 10-5903-02 | 0.25g | 875.00 | |
| 5’-Dichloro-dimethoxy-Fluorescein Phosphoramidite | 10-5904-95 | 50 µmole | 175.00 |
| (JOE) | 10-5904-90 | 100 µmole | 340.00 |
| 10-5904-02 | 0.25g | 775.00 | |
| Fluorescein Phosphoramidite | 10-1963-95 | 50 µmole | 165.00 |
| 10-1963-90 | 100 µmole | 295.00 | |
| 10-1963-02 | 0.25g | 095.00 | |
| 6-Fluorescein Phosphoramidite | 10-1964-95 | 50 µmole | 165.00 |
| 10-1964-90 | 100 µmole | 295.00 | |
| 10-1964-02 | 0.25g | 595.00 | 6-Fluorescein Serinol Phosphoramidite | 10-1994-95 | 50 µmole | 165.00 |
| 10-1994-90 | 100 µmole | 295.00 | |
| 10-1994-02 | 0.25g | 595.00 | |
| Fluorescein-dT Phosphoramidite | 10-1056-95 | 50 µmole | 180.00 |
| 10-1056-90 | 100 µmole | 325.00 | |
| 10-1056-02 | 0.25g | 675.00 | |
| 3'-Fluorescein CPG | 20-2963-01 | 0.1g | 120.00 |
| 20-2963-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-2963-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-2963-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-2963-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2963-14 | Pack of 1 | 450.00 |
| 3'-(6-Fluorescein) CPG | 20-2964-01 | 0.1g | 120.00 |
| 20-2964-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-2964-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-2964-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-2964-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2964-14 | Pack of 1 | 450.00 |
| 3'-(6-FAM) CPG | 20-2961-01 | 0.1g | 120.00 |
| 20-2961-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-2961-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-2961-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-2961-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2961-14 | Pack of 1 | 450.00 |
| 3'-(6-FAM) PS | |||
| 200 nmole columns (ABI 3900) | 26-2961-52 | Pack of 10 | 300.00 |
| 40 nmole columns (ABI 3900) | 26-2961-55 | Pack of 10 | 300.00 |
| 3’-6-Fluorescein Serinol CPG | 20-2994-01 | 0.1g | 120.00 |
| 20-2994-10 | 1.0g | 995.00 | |
| 0.2 µmole columns | 20-2994-42 | Pack of 4 | 120.00 |
| 1 µmole columns | 20-2994-41 | Pack of 4 | 200.00 |
| 10 µmole column (ABI) | 20-2994-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2994-14 | Pack of 1 | 450.00 |
| 3'-Fluorescein-dT CPG | 20-2056-01 | 0.1g | 120.00 |
| 20-2056-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-2056-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-2056-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-2056-13 | Pack of 1 | 300.00 |
| 15 µmole column (Expedite) | 20-2056-14 | Pack of 1 | 450.00 |
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
Absorbance Maximum |
Emission Maximum |
Color |
|
Fluorescein |
494nm |
525nm |
Green |
Tetrachloro Fluorescein |
521nm |
536nm |
Orange |
Hexachloro Fluorescein |
535nm |
556nm |
Pink |
TAMRA |
565nm |
580nm |
Rose |
Cy3 |
546nm |
563nm |
Red |
Cy3.5 |
588nm |
604nm |
Purple |
Cy5 |
646nm |
662nm |
Violet |
Cy5.5 |
683nm |
707nm |
Dark Blue |
Yakima Yellow |
530nm |
549nm |
Yellow |
Redmond Red |
579nm |
595nm |
Red |
Spectral Characteristics of Joe | |||
| E260 | 12,000 L/mol.cm | Absorbance | 525 nm |
| Emax | 75,000 L/mol.cm | Emission | 548 nm |
| QY | 0.58 | ||
Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3’-TAMRA CPG for 3’ labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.
| Item | Catalog No. | Pack | Price ($) |
| 3'-TAMRA CPG | 20-5910-01 | 0.1g | 120.00 |
| 20-5910-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-5910-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-5910-42 | Pack of 4 | 120.00 |
| 3'-Tamra PS | |||
| 200 nmole columns (AB 3900) | 26-5910-52 | Pack of 10 | 300.00 |
| 40 nmole columns (AB 3900) | 26-5910-55 | Pack of 10 | 300.00 |
| TAMRA-dT | 10-1057-95 | 50 µmole | 250.00 |
| 10-1057-90 | 100 µmole | 495.00 | |
| 10-1057-02 | 0.25g | 975.00 | |
| TAMRA NHS Ester | 00-5910-66 | 60 µL | 200.00 |
| (Solution in anhydrous DMSO) | |||
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
Two cyanine derivatives, Cy3™ and Cy5™, which differ in structure simply by the number of carbons in the conjugated poly-ene linkage, are joined by the closely related analogues, Cy3.5™ and Cy5.5™, and are available as phosphoramidites. Cy dyes are normally added once at the 5’-terminus and the MMT group should be removed on the synthesizer. The absorbance of the MMT cation (yellow) is noticeably different from the DMT cation (orange), and so, absorbance-based trityl monitors will detect it incorrectly as a low coupling. On the other hand, conductivity detectors will interpret the release more correctly. Cy dyes have also been used successfully adjacent to the 3’-terminus as long as 0.02M iodine oxidizer is used during the remainder of the synthesis.
Deprotection of oligos containing Cy dyes may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. If there is a need to use ammonium hydroxide at elevated temperature, Cy3 and Cy 3.5 are more stable than Cy5 and Cy5.5. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 55°C.
| Item | Catalog No. | Pack | Price ($) |
| Cy3 Phosphoramidite | 10-5913-95 | 50 µmole | 190.00 |
| 10-5913-90 | 100 µmole | 350.00 | |
| 10-5913-02 | 0.25g | 875.00 | |
| Cy3.5 Phosphoramidite | 10-5914-95 | 50 µmole | 345.00 |
| 10-5914-90 | 100 µmole | 660.00 | |
| 10-5914-02 | 0.25g | 1550.00 | |
| Cy5 Phosphoramidite | 10-5915-95 | 50 µmole | 190.00 |
| 10-5915-90 | 100 µmole | 350.00 | |
| 10-5915-02 | 0.25g | 875.00 | |
| Cy5.5 Phosphoramidite | 10-5916-95 | 50 µmole | 225.00 |
| 10-5916-90 | 100 µmole | 420.00 | |
| 10-5916-02 | 0.25g | 875.00 |
|
Absorbance Maximum |
Emission Maximum |
Color | |
| Cy3 |
546nm |
563nm |
Red |
| Cy3.5 |
588nm |
604nm |
Purple |
| Cy5 |
646nm |
662nm |
Violet |
| Cy5.5 |
683nm |
707nm |
Dark Blue |
|
(Measured in an oligo in 0.1M TEAA buffer, pH7.) | |||
Cyanine Dyes
This material is subject to proprietary rights of Amersham Biosciences Corp. and is made and sold under license from Amersham Biosciences Corp. under the following patents: US Patent No. 5,556,959 and 5,808,044 and other patents pending. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use.
Cy and Cy dye are trademarks of Amersham Biosciences Corp.
Glen Research’s agreement with Epoch Biosciences, Inc., a subsidiary of Nanogen, Inc., allows us to sell several of Epoch’s proprietary products designed for the synthesis of novel DNA probes. We are pleased to offer products based on Epoch’s Redmond Red™, Yakima Yellow™ and Gig Harbor Green™ fluorophores and Eclipse™ non-fluorescent quencher. Under our agreement we also supply PPG, a modified nucleoside and 5’-Aldehyde-Modifier C2 Phosphoramidite. The two fluorescent dyes, Yakima Yellow and Redmond Red, are available as phosphoramidites and supports. Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, while Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively. Gig Harbor Green and 6-FAM are based on the same fluorescein core structure but Gig Harbor Green is 15-20% brighter than FAM.
The Eclipse quencher from Epoch solves most of the problems inherent in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.
| Item | Catalog No. | Pack | Price ($) |
| Epoch Redmond Red Phosphoramidite | 10-5920-95 | 50 µmole | 205.00 |
| 10-5920-90 | 100 µmole | 410.00 | |
| 10-5920-02 | 0.25g | 995.00 | |
| Epoch Yakima Yellow Phosphoramidite | 10-5921-95 | 50 µmole | 205.00 |
| 10-5921-90 | 100 µmole | 410.00 | |
| 10-5921-02 | 0.25g | 995.00 | |
| Epoch Gig Harbor Green Phosphoramidite | 10-5922-95 | 50 µmole | 165.00 |
| 10-5922-90 | 100 µmole | 325.00 | |
| 10-5922-02 | 0.25g | 875.00 | |
| Epoch Eclipse Quencher Phosphoramidite | 10-5925-95 | 50 µmole | 225.00 |
| 10-5925-90 | 100 µmole | 450.00 | |
| 10-5925-02 | 0.25g | 1125.00 | |
| Epoch Redmond Red CPG | 20-5920-01 | 0.1g | 180.00 |
| 20-5920-10 | 1.0g | 1500.00 | |
| 20-5920-41 | Pack of 4 | 300.00 | |
| 20-5920-42 | Pack of 4 | 150.00 | |
| 20-5920-13 | Pack of 1 | 750.00 | |
| 20-5920-14 | Pack of 1 | 1125.00 | |
| Epoch Yakima Yellow CPG | 20-5921-01 | 0.1g | 180.00 |
| 20-5921-10 | 1.0g | 1500.00 | |
| 20-5921-41 | Pack of 4 | 300.00 | |
| 20-5921-42 | Pack of 4 | 150.00 | |
| 20-5921-13 | Pack of 1 | 750.00 | |
| 20-5921-14 | Pack of 1 | 1125.00 | |
| Epoch Eclipse QuencherCPG | 20-5925-01 | 0.1g | 230.00 |
| 20-5925-10 | 1.0g | 1925.00 | |
| 20-5925-41 | Pack of 4 | 350.00 | |
| 20-5925-42 | Pack of 4 | 175.00 | |
| 20-5925-13 | Pack of 1 | 995.00 | |
| 20-5925-14 | Pack of 1 | 1495.00 |
PPG p50
Absorbance Maximum |
Emission Maximum |
Color |
|
Gig Harbor Green |
494nm |
525nm |
Green |
Yakima Yellow |
530nm |
549nm |
Yellow |
Redmond Red |
579nm |
595nm |
Red |
These Products are for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Products are subject to proprietary rights of Epoch Biosciences, Inc. and are made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to the Products and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of the Products. “Commercial use” includes but is not limited to the sale, lease, license or other transfer of the Products or any material derived or produced from them, the sale, lease, license or other grant of rights to use the Products or any material derived or produced from them, or the use of the Products to perform services for a fee for third parties (including contract research).
A simple agreement must be signed before end-users and custom oligo services may purchase these products for use as defined above.
http://www.glenresearch.com/
Reference/EpochProducts.pdf
Redmond Red, Yakima Yellow, Gig Harbor Green, and Eclipse are trademarks of Epoch Biosciences, Inc.
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
With the growing popularity of red and near-infrared dyes, we are offering the Black Hole QuencherTM dyes (BHQs), whose physical properties are detailed in Table 1. BHQ dyes are robust dark quenchers that very nicely complement our existing product line. They are compatible with ammonium hydroxide deprotection, exhibit excellent coupling efficiencies, have large extinction coefficients and are completely non-fluorescent. Their absorbances are well-tuned to quench a variety of popular fluorophores – even those far into the red, such as Cy3 and Cy5. The dark quencher most typically used in a Molecular Beacon is Dabcyl. Because the quenching does not involve FRET, there is little, if any, dependence upon donor-acceptor spectral overlap. In a comprehensive paper by Marras, Kramer and Tyagi,1 the ability of BHQ-1 and BHQ-2 to quench 22 different fluorophores was evaluated. For shorter wavelength fluorophores such as fluorescein, the quenching efficiency was roughly the same as Dabcyl (91% – 93%). However, for dyes emitting in the far red, such as Cy5, the BHQ dyes were far superior – quenching the Cy5 with 96% efficiency, compared to 84% with Dabcyl. This may reflect the BHQ’s ability to form stable, non-fluorescent complexes which can be a plus even in FRET probes. Indeed, recent work suggests that these non-fluorescent complexes will form even in the absence of a hairpin stem structure used by Molecular Beacons.2
| Item | Catalog No. | Pack | Price ($) |
| 5'-BHQ-0 Phosphoramidite | 10-5930 | Discontinued | |
| (Discontinued. Replacement is 5’-Dabcyl Phosphoramidite (10-5912) or 10-5931 below.) | |||
| 5'-BHQ-1 Phosphoramidite | 10-5931-95 | 50 µmole | 100.00 |
| 10-5931-90 | 100 µmole | 200.00 | |
| 10-5931-02 | 0.25g | 700.00 | |
| 5'-BHQ-2 Phosphoramidite | 10-5932-95 | 50 µmole | 100.00 |
| 10-5932-90 | 100 µmole | 200.00 | |
| 10-5932-02 | 0.25g | 700.00 | |
| BHQ-1-dT | 10-5941-95 | 50 µmole | 265.00 |
| 10-5941-90 | 100 µmole | 025.00 | |
| 10-5941-02 | 0.25g | 925.00 | |
| BHQ-2-dT | 10-5942-95 | 50 µmole | 265.00 |
| 10-5942-90 | 100 µmole | 025.00 | |
| 10-5942-02 | 0.25g | 925.00 | |
| 3'-BHQ-0 CPG | 20-5930 | Discontinued | |
| (Discontinued. Replacement is 3’-Dabcyl CPG (20-5912) or 20-5931 below.) | |||
| 15 µmole column (Expeidite) | 20-5930-14 | Pack of 1 | 825.00 |
| 3'-BHQ-1 CPG | 20-5931-01 | 0.1g | 190.00 |
| 20-5931-10 | 1.0g | 1500.00 | |
| 1 µmole columns | 20-5931-41 | Pack of 4 | 300.00 |
| 0.2 µmole columns | 20-5931-42 | Pack of 4 | 80.00 |
| 10 µmole column (ABI) | 20-5931-13 | Pack of 1 | 075.00 |
| 15 µmole column (Expeidite) | 20-5931-14 | Pack of 1 | 825.00 |
| 3'-BHQ-2 CPG | 20-5932-01 | 0.1g | 190.00 |
| 20-5932-10 | 1.0g | 1500.00 | |
| 1 µmole columns | 20-5932-41 | Pack of 4 | 300.00 |
| 0.2 µmole columns | 20-5932-42 | Pack of 4 | 80.00 |
| 10 µmole column (ABI) | 20-5932-13 | Pack of 1 | 075.00 |
| 15 µmole column (Expeidite) | 20-5932-14 | Pack of 1 | 825.00 |
| 3'-BHQ-3 CPG | 20-5933-01 | 0.1g | 190.00 |
| 20-5933-10 | 1.0g | 1500.00 | |
| 1 µmole columns | 20-5933-41 | Pack of 4 | 300.00 |
| 0.2 µmole columns | 20-5933-42 | Pack of 4 | 80.00 |
| 10 µmole column (ABI) | 20-5933-13 | Pack of 1 | 075.00 |
| 15 µmole column (Expeidite) | 20-5933-14 | Pack of 1 | 825.00 |
| Quencher | lmax (nm) |
E260 (L/mol.cm) |
Emax (L/mol.cm) |
BHQ-0 |
493 | 7,700 | 34,000 |
BHQ-1 |
534 | 8,000 | 34,000 |
BHQ-2 |
579 | 8,000 | 38,000 |
BHQ-3 |
672 | 13,000 | 42,700 |
(1) S.A.E. Marras, F.R. Kramer, and S. Tyagi, Nucleic Acids Res., 2002, 30, E122.
(2) M.K. Johansson, H. Fidder, D. Dick, and R.M. Cook, J Am Chem Soc, 2002, 124, 6950-6956.
"Black Hole Quencher", "BHQ-0", "BHQ-1", "BHQ-2" and "BHQ-3" are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3’-terminus. Acridine is an effective intercalating agent.
| Item | Catalog No. | Pack | Price ($) |
| Acridine Phosphoramidite | 10-1973-95 | 50 µmole | 165.00 |
| 10-1973-90 | 100 µmole | 295.00 | |
| 10-1973-02 | 0.25g | 675.00 | |
| 3'-Acridine CPG | 20-2973-01 | 0.1g | 120.00 |
| 20-2973-10 | 1.0g | 995.00 | |
| 1 µmole columns | 20-2973-41 | Pack of 4 | 200.00 |
| 0.2 µmole columns | 20-2973-42 | Pack of 4 | 120.00 |
| 10 µmole column (ABI) | 20-2973-13 | Pack of 1 | 300.00 |
| 15 µmole cloumn (Expedite) | 20-2973-14 | Pack of 1 | 450.00 |
All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.
Monomers |
||
For Instrument type |
Add |
|
Expedite |
E |
|
Beckman Oligo 1000 |
B |
|
Pharmacia Gene Assembler |
P |
|
Mermade |
M |
|
Columns |
||
For Instrument type |
Add |
|
Applied Biosystems 3900 |
A |
|
Expedite |
E |
|
Mermade |
M |
|
Please inquire for availability
of columns for other instrument types. |
||
An analytical test based on detection of 2,4-dinitrophenyl (DNP) labelled oligonucleotides with anti-DNP antibodies has been proposed. We have chosen the branched triethylene glycol (TEG) spacer in our version of DNP phosphoramidite since it can be added once or several times to the 3’ or 5’ terminus.
| Item | Catalog No. | Pack | Price ($) |
| DNP-TEG Phosphoramidite | 10-1985-95 | 50 µmole | 165.00 |
| 10-1985-90 | 100 µmole | 295.00 | |
| 10-1985-02 | 0.25g | 675.00 |
Potential therapeutic oligonucleotides must permeate the cell membrane for optimal activity. The addition of lipophilic groups to an oligonucleotide would be expected to enhance activity. The use of cholesteryl oligos and the consequent improvement in activity has been described. We have designed our Cholesteryl Phosphoramidite with our branched triethyleneglycol (TEG) spacer for maximum solubility in acetonitrile as well as for applications requiring multiple labels.
ABI-style vials and columns are supplied unless otherwise requested (see note box).
| Item | Catalog No. | Pack | Price ($) |