Spectral Characteristics of Fluorescent Dyes

Absorbance Maximum Emission Maximum Color
Fluorescein 494nm 525nm Green
Tetrachloro-Fluorescein 521nm 536nm Orange
Hexachloro-Fluorescein 535nm 556nm Pink
SIMA (HEX) 538nm 551nm Pink
Dichloro-dimethoxy-Fluorescein 525nm 548nm Orange/Pink
TAMRA 565nm 580nm Rose
Cy3 546nm 563nm Red
Cy3.5 588nm 604nm Purple
Cy5 646nm 662nm Violet
Cy5.5 683nm 707nm Dark Blue
Yakima Yellow 530nm 549nm Yellow
Redmond Red 579nm 595nm Red

 


 

Dye Quencher Plot