Ultramild DNA Synthesis (Catalog as PDF)

Ultramild CE Phosphoramidites

The synthesis of labelled oligonucleotides has become a standard procedure in many laboratories and many labelling reagents, e.g., biotin and fluorescein, are now available as β-cyanoethyl (CE) phosphoramidites. Labels which are currently available as CE phosphoramidites have one common property - they must be stable to the strongly alkaline conditions required for removal of the base protecting groups. This property is lacking in several interesting dyes and labels. We sought an alternative protecting scheme for the normal CE phosphoramidites which would allow UltraMILD deprotection and would not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.
Item Catalog No. Pack Price ($)
10-1601-02 0.25g 15.00
10-1601-05 0.5g 30.00
10-1601-10 1.0g 60.00
10-1015-02 0.25g 12.50
10-1015-05 0.5g 25.00
10-1015-10 1.0g 50.00
10-1621-02 0.25g 15.00
10-1621-05 0.5g 30.00
10-1621-10 1.0g 60.00
Pac-dA
Pac-dA
iPr-Pac-dG
iPr-Pac-dG
Ac-dC
Ac-dC

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Ultramild Supports

Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
Item Pac-dA Ac-dC iPr-Pac-dG    
20-2601-01 Listed 20-2621-01 0.1g 18.00
20-2601-02 on 20-2621-02 0.25g 40.00
20-2601-10 Page 8 20-2621-10 1.0g 150.00
ABI Columns
40 nmole columns 20-2701-45 20-2115-45 20-2721-45 4x40nm 40.00
0.2 µmole columns 20-2701-42 20-2115-42 20-2721-42 4x0.2µm 40.00
1 µmole columns 20-2701-41 20-2115-41 20-2721-41 4x1.0µm 60.00
10 µmole column (ABI) 20-2701-13 20-2115-13 20-2721-13 1x10µm 100.00
Expedite Columns
40 nmole columns 20-2801-45 20-2215-45 20-2821-45 4x40nm 40.00
0.2 µmole columns 20-2801-42 20-2215-42 20-2821-42 4x0.2µm 40.00
1 µmole columns 20-2801-41 20-2215-41 20-2821-41 4x1.0µm 60.00
15 µmole column (Expedite) 20-2801-14 20-2215-14 20-2821-14 1x15µm 150.00
Pac-dA-CPG
Pac-dA-CPG
iPr-Pac-dG-CPG
iPr-Pac-dG-CPG
Ac-dC-CPG 1000
Ac-dC-CPG 1000

See Also

Ultramild Solvents/Reagents

Item Catalog No. Pack Price ($)
40-4210-52 200mL 140.00
40-4210-57 450mL 300.00
40-4212-52 200mL 140.00
40-4212-57 450mL 300.00
60-4600-30 30mL 30.00

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