Ultramild DNA Synthesis (Catalog as PDF)

Ultramild CE Phosphoramidites

An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.
Item Catalog No. Pack Price ($)
10-1601-02 0.25g 15.00
10-1601-05 0.5g 30.00
10-1601-10 1.0g 60.00
10-1015-02 0.25g 12.50
10-1015-05 0.5g 25.00
10-1015-10 1.0g 50.00
10-1621-02 0.25g 15.00
10-1621-05 0.5g 30.00
10-1621-10 1.0g 60.00
Pac-dA
Pac-dA
iPr-Pac-dG
iPr-Pac-dG
Ac-dC
Ac-dC

Ultramild Supports

Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
Item Pac-dA Ac-dC iPr-Pac-dG    
UltraMild CPG (Bulk)
20-2601-01 Listed 20-2621-01 0.1g 18.00
20-2601-02 on 20-2621-02 0.25g 40.00
20-2601-10 Page 8 20-2621-10 1.0g 150.00
ABI Columns
40 nmole columns 20-2701-45 20-2115-45 20-2721-45 4x40nm 40.00
0.2 µmole columns 20-2701-42 20-2115-42 20-2721-42 4x0.2µm 40.00
1 µmole columns 20-2701-41 20-2115-41 20-2721-41 4x1.0µm 60.00
10 µmole column (ABI) 20-2701-13 20-2115-13 20-2721-13 1x10µm 100.00
Expedite Columns
40 nmole columns 20-2801-45 20-2215-45 20-2821-45 4x40nm 40.00
0.2 µmole columns 20-2801-42 20-2215-42 20-2821-42 4x0.2µm 40.00
1 µmole columns 20-2801-41 20-2215-41 20-2821-41 4x1.0µm 60.00
15 µmole column (Expedite) 20-2801-14 20-2215-14 20-2821-14 1x15µm 150.00
Pac-dA-CPG
Pac-dA-CPG
iPr-Pac-dG-CPG
iPr-Pac-dG-CPG
Ac-dC-CPG 1000
Ac-dC-CPG 1000

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

Ultramild Solvents/Reagents

Item Catalog No. Pack Price ($)
Cap Mix A
40-4210-52 200mL 140.00
  (Applied Biosystems) 40-4210-57 450mL 300.00
40-4212-52 200mL 140.00
  (Expedite) 40-4212-57 450mL 300.00
Deprotection Solution
60-4600-30 30mL 30.00

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