2'-OMe-RNA Synthesis (Catalog as PDF)

2'-OMe-RNA Phosphoramidites

Glen Research 2'-OMe-RNA CE (β-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.
Item Catalog No. Pack Price ($)
10-3100-90 100µm 20.00
10-3100-02 0.25g 50.00
10-3100-05 0.5g 100.00
10-3100-10 1.0g 200.00
10-3110-90 100µm Discontinued
10-3110-02 0.25g Discontinued
10-3110-05 0.5g 100.00
10-3110-10 1.0g 200.00
10-3115-90 100µm 20.00
10-3115-02 0.25g 50.00
10-3115-05 0.5g 100.00
10-3115-10 1.0g 200.00
10-3120-90 100µm 20.00
10-3120-02 0.25g 50.00
10-3120-05 0.5g 100.00
10-3120-10 1.0g 200.00
10-3121-90 100µm 20.00
10-3121-02 0.25g 50.00
10-3121-05 0.5g 100.00
10-3121-10 1.0g 200.00
10-3130-90 100µm 20.00
10-3130-02 0.25g 50.00
10-3130-05 0.5g 100.00
10-3130-10 1.0g 200.00
2'-OMe-A
2'-OMe-A
2'-OMe-Ac-C
2'-OMe-Ac-C
2'-OMe-ibu-G
2'-OMe-ibu-G
2'-OMe-G
2'-OMe-G
2'-OMe-U
2'-OMe-U

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

HT 2'-OMe-RNA Phosphoramidites

Item Catalog No. Pack Price ($)
10-3100-2HT 2g 400.00
10-3115-2HT 2g 400.00
10-3120-2HT 2g 400.00
10-3130-2HT 2g 400.00
2'-OMe-A
2'-OMe-A
2'-OMe-Ac-C
2'-OMe-Ac-C
2'-OMe-ibu-G
2'-OMe-ibu-G
2'-OMe-U
2'-OMe-U

HT 2'-OMe-RNA

Minimum order: 40g total

Products may be combined to 40g total. Please request a quote.

Ultramild 2'-OMe-RNA

The use of UltraMild monomers in oligonucleotide synthesis has allowed very sensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely. The DNA and RNA monomers are currently available and we also provide this set of 2'-OMe-RNA monomers. In our version of this chemistry, we use as protecting groups phenoxyacetyl (Pac) for A, acetyl (Ac) for C, and isopropyl-phenoxyacetyl (iPr-Pac) for G.

It has become clear that acetic anhydride in the conventional capping mix can cause transamidation in situations where an amine protecting group is quite labile. This leads to acetyl protection on the amino group that may be slow to be removed. Consequently, if many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix.
Item Catalog No. Pack Price ($)
10-3601-90 100µm 25.00
10-3601-02 0.25g 62.50
10-3601-05 0.5g 125.00
10-3601-10 1.0g 250.00
10-3115-90 100µm 20.00
10-3115-02 0.25g 50.00
10-3115-05 0.5g 100.00
10-3115-10 1.0g 200.00
10-3621-90 100µm 25.00
10-3621-02 0.25g 62.50
10-3621-05 0.5g 125.00
10-3621-10 1.0g 250.00
2'-OMe-Pac-A
2'-OMe-Pac-A
2'-OMe-Ac-C
2'-OMe-Ac-C
2'-OMe-iPr-Pac-G
2'-OMe-iPr-Pac-G

Ultramild Solvents/Reagents

Item Catalog No. Pack Price ($)
Cap Mix A
40-4210-52 200mL 140.00
  (Applied Biosystems) 40-4210-57 450mL 300.00
40-4212-52 200mL 140.00
  (Expedite) 40-4212-57 450mL 300.00
Deprotection Solution
60-4600-30 30mL 30.00

2'-OMe-RNA Supports

ABI-style columns are supplied for 1 µmole and 0.2 µmole scales unless otherwise requested (see note box).
Item Catalog No. Pack Price ($)
20-3600-01 0.1g 40.00
20-3600-02 0.25g 95.00
20-3600-10 1.0g 355.00
  1 µmole columns 20-3700-41 Pack of 4 100.00
  0.2 µmole columns 20-3700-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3700-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3700-14 Pack of 1 300.00
20-3610-01 0.1g 40.00
20-3610-02 0.25g 95.00
20-3610-10 1.0g 355.00
  1 µmole columns 20-3710-41 Pack of 4 100.00
  0.2 µmole columns 20-3710-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3710-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3710-14 Pack of 1 300.00
20-3615-01 0.1g 40.00
20-3615-02 0.25g 95.00
20-3615-10 1.0g 355.00
  1 µmole columns 20-3715-41 Pack of 4 100.00
  0.2 µmole columns 20-3715-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3715-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3715-14 Pack of 1 300.00
20-3621-01 0.1g 40.00
20-3621-02 0.25g 95.00
20-3621-10 1.0g 355.00
  1 µmole columns 20-3721-41 Pack of 4 100.00
  0.2 µmole columns 20-3721-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3721-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3721-14 Pack of 1 300.00
20-3630-01 0.1g 40.00
20-3630-02 0.25g 95.00
20-3630-10 1.0g 355.00
  1 µmole columns 20-3730-41 Pack of 4 100.00
  0.2 µmole columns 20-3730-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3730-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3730-14 Pack of 1 300.00
2'-OMe-A-RNA-CPG
2'-OMe-A-RNA-CPG
2'-OMe-C-RNA-CPG
2'-OMe-C-RNA-CPG
2'-OMe-Ac-C-RNA-CPG
2'-OMe-Ac-C-RNA-CPG
2'-OMe-G-RNA-CPG
2'-OMe-G-RNA-CPG
2'-OMe-U-RNA-CPG
2'-OMe-U-RNA-CPG

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Minor 2'-OMe-RNA Phosphoramidites

To aid in the evaluation of the structures of 2'-OMe-RNA complexes, we offer the CE phosphoramidites listed below. 2'-OMe-T is useful in triplex studies while the 2-aminopurine derivative may be tested in ribozyme studies. By supporting an additional hydrogen bond, 2,6-diaminopurine (2-amino-adenosine) binds more strongly with uridine than does adenosine. Oligonucleotides containing 2'-OMe-5-Me-C and 2'-OMe-I would be of interest to researchers involved in triplex and antisense studies using 2'-OMe-RNA. The uses of 2'-OMe-5-bromo-U phosphoramidite range from crystallographic studies due to the heavy atom to cross-linking because of its photolability. 5-Fluoro-pyrimidine nucleosides have been useful as therapeutic agents and their effect on the structure and activity of oligonucleotides may be examined using the 2'-OMe-RNA derivatives. The 2,4,6-trimethylphenyl (TMP) protected 2'-OMe-U derivative is a convertible nucleoside and reaction with ammonia leads to the 5-fluoro-dC analogue. 2'-OMe-3-deaza-5-aza-C (Reverse C) derivative has the potential to mimic in oligonucleotides 5-azacytidine, a DNA methylase inhibitor. Its ability to bind as a C will likely be diminished.

ABI-style vials are supplied unless otherwise requested (see note box).
Item Catalog No. Pack Price ($)
10-3123-95 50µm 177.50
  (N-dmf-AP) 10-3123-90 100µm 355.00
10-3123-02 0.25g 975.00
10-3124-95 50µm 177.50
  (2-amino-A) 10-3124-90 100µm 355.00
10-3124-02 0.25g 975.00
10-3131-90 100µm 150.00
  (2’-OMe-T) 10-3131-02 0.25g 360.00
10-3140-90 100µm 150.00
10-3140-02 0.25g 360.00
10-3160-90 100µm 240.00
10-3160-02 0.25g 675.00
10-3190-90 100µm 240.00
10-3190-02 0.25g 675.00
10-3111-95 50µm 177.50
  (2’-OMe-5-F-C Precursor) 10-3111-90 100µm 355.00
10-3111-02 0.25g 975.00
10-3132-95 50µm 177.50
10-3132-90 100µm 355.00
10-3132-02 0.25g 975.00
10-3116-95 50µm Discontinued
10-3116-90 100µm 355.00
10-3116-02 0.25g 975.00
2'-OMe-2-Aminopurine
2'-OMe-2-Aminopurine
2'-OMe-2,6-Diaminopurine
2'-OMe-2,6-Diaminopurine
2'-OMe-5-Me-U
2'-OMe-5-Me-U
2'-OMe-I
2'-OMe-I
2'-OMe-5-Me-C
2'-OMe-5-Me-C
2'-OMe-5-Br-U
2'-OMe-5-Br-U
2'-OMe-TMP-5-F-U
2'-OMe-TMP-5-F-U
2'-OMe-5-F-U
2'-OMe-5-F-U

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

2’-OMe-Thiophosphoramidites

The phosphorodithioate linkage (PS2) is both achiral and essentially resistant to nucleases. Previous studies have shown very interesting results which include observations that DNA with PS2 linkages activates RNase H in vitro, strongly inhibits human immunodeficiency virus (HIV) reverse transcriptase, induces β-cell proliferation and differentiation, and is completely resistant to hydrolysis by various nucleases. 2'-OMe- RNA Thiophosphoramidites are RNA monomers designed to produce oligos combining the PS2 linkage with the 2'-O-methyl ribose modification. These PS2-modified RNA oligos have potential for use in siRNAs and dithiophosphate aptamers (thioaptamers).
Item Catalog No. Pack Price ($)
10-3170-90 100µm 300.00
10-3170-02 0.25g 720.00
10-3171-90 100µm 300.00
10-3171-02 0.25g 720.00
10-3172-90 100µm 300.00
10-3172-02 0.25g 720.00
10-3173-90 100µm 300.00
10-3173-02 0.25g 720.00
2'-OMe-A-Thiophosphoramidite
2'-OMe-A-Thiophosphoramidite
2’-OMe-C-Thiophosphoramidite
2’-OMe-C-Thiophosphoramidite
2’-OMe-G-Thiophosphoramidite
2’-OMe-G-Thiophosphoramidite
2’-OMe-U-Thiophosphoramidite
2’-OMe-U-Thiophosphoramidite

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019