2'-F-RNA Monomers (Catalog as PDF)

2'-F-RNA Monomers

2'-Deoxy-2'-fluoro-nucleosides adopt an RNA-type sugar conformation, presumably due to the high electronegativity of fluorine. Because of this sugar conformation, RNA duplexes (A-form) are generally more thermodynamically stable than DNA duplexes (β-form). As expected, the addition of 2'-F-RNA residues to oligodeoxynucleotides progressively increases the thermal stability of their duplexes with RNA. The stabilization is additive at approximately 2° per residue. This compares favorably with 2'-OMe-RNA at around 1.5° and RNA at 1.1° per residue. In the meantime, base pair specificity remains intact.

2'-F-RNA phosphodiester linkages are not nuclease resistant, although the corresponding phosphorothioate linkages are highly resistant. Researchers usually design antisense oligonucleotides to form duplexes with RNA, which are then substrates for RNase H. Uniformly modified 2'-F-RNA/RNA duplexes are not substrates for RNase H. However, it is straightforward to prepare chimeric 2'-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant.
Item Catalog No. Pack Price ($)
10-3400-02 0.25g 100.00
10-3400-05 0.5g 200.00
10-3415-02 0.25g 50.00
10-3415-05 0.5g 100.00
10-3420-02 0.25g 100.00
10-3420-05 0.5g 200.00
10-3430-02 0.25g 50.00
10-3430-05 0.5g 100.00
2'-F-A
2'-F-A
2'-F-Ac-C
2'-F-Ac-C
2'-F-G
2'-F-G
2'-F-U
2'-F-U

STABILITY NOTE

Synthetic oligonucleotides containing 2'-F-RNA linkages may be deprotected with ammonium hydroxide as normal. Deprotection using AMA at 65°C leads to some degradation and so we recommend the use of AMA at room temperature for 2 hours.

Intellectual Property

A license may be required from Ionis Pharmaceuticals, Inc. to incorporate 2'-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 6,005,087; 6,531,584 and foreign equivalents.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

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Applied Biosystems 3900
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