RNA Synthesis (Catalog as PDF)

TOM-Protected RNA Phosphoramidites

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.
Item Catalog No. Pack Price ($)
10-3004-02 0.25g 75.00
10-3004-05 0.5g 150.00
10-3004-10 1.0g 275.00
10-3014-02 0.25g 75.00
10-3014-05 0.5g 150.00
10-3014-10 1.0g 275.00
10-3024-02 0.25g 75.00
10-3024-05 0.5g 150.00
10-3024-10 1.0g 275.00
10-3034-02 0.25g 75.00
10-3034-05 0.5g 150.00
10-3034-10 1.0g 275.00
A-TOM
A-TOM
C-TOM
C-TOM
G-TOM
G-TOM
U-TOM
U-TOM

Intellectual Property

Tom Group TOM-Protecting-Group™

TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.

TOM-Protecting-Group is a trademark of QIAGEN.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

RNA Supports for TOM RNA Synthesis

Item Catalog No. Pack Price ($)
20-3304-01 0.1g 40.00
20-3304-02 0.25g 95.00
20-3304-10 1.0g 355.00
  1 µmole columns 20-3404-41 Pack of 4 100.00
  0.2 µmole columns 20-3404-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3404-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3404-14 Pack of 1 300.00
20-3315-01 0.1g 40.00
20-3315-02 0.25g 95.00
20-3315-10 1.0g 355.00
  1 µmole columns 20-3415-41 Pack of 4 100.00
  0.2 µmole columns 20-3415-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3415-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3415-14 Pack of 1 300.00
20-3324-01 0.1g 40.00
20-3324-02 0.25g 95.00
20-3324-10 1.0g 355.00
  1 µmole columns 20-3424-41 Pack of 4 100.00
  0.2 µmole columns 20-3424-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3424-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3424-14 Pack of 1 300.00
20-3330-01 0.1g 40.00
20-3330-02 0.25g 95.00
20-3330-10 1.0g 355.00
  1 µmole columns 20-3430-41 Pack of 4 100.00
  0.2 µmole columns 20-3430-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3430-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3430-14 Pack of 1 300.00
Ac-A-RNA-CPG
Ac-A-RNA-CPG
Ac-C-RNA-CPG
Ac-C-RNA-CPG
Ac-G-RNA-CPG
Ac-G-RNA-CPG
U-RNA-CPG
U-RNA-CPG

RNA Supports for TOM RNA Synthesis (Cont.)

Item Catalog No. Pack Price ($)
10-3003-02 0.25g 40.00
10-3003-05 0.5g 80.00
10-3003-10 1.0g 160.00
10-3015-02 0.25g 40.00
10-3015-05 0.5g 80.00
10-3015-10 1.0g 160.00
10-3025-02 0.25g 40.00
10-3025-05 0.5g 80.00
10-3025-10 1.0g 160.00
10-3030-02 0.25g 40.00
10-3030-05 0.5g 80.00
10-3030-10 1.0g 160.00
Bz-A
Bz-A
Ac-C
Ac-C
Ac-G
Ac-G
U
U

TBDMS-Protected RNA Phosphoramidites

Glen Research CE (β-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).
Item Catalog No. Pack Price ($)
10-3003-2HT 2g 320.00
10-3015-2HT 2g 320.00
10-3025-2HT 2g 320.00
10-3030-2HT 2g 320.00
Bz-A
Bz-A
Ac-C
Ac-C
Ac-G
Ac-G
U
U

ABBREVIATIONS

Bz = Benzoyl
CNEt = Cyanoethyl
CPG = Controlled Pore Glass
dmf = Dimethylformamidine
DMT = 4,4'-Dimethoxytrityl
iPr = Isopropyl
lcaa = long chain alkylamino
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TBDMS = t-Butyl-dimethylsilyl

HT RNA Phosphoramidites

Item Catalog No. Pack Price ($)
10-3003-10 1.0g 160.00
10-3015-10 1.0g 160.00
10-3025-10 1.0g 160.00
10-3030-10 1.0g 160.00
Bz-A
Bz-A
Ac-C
Ac-C
Ac-G
Ac-G
U
U

HT RNA

Minimum order: 40g total Products may be combined to 40g total. Please request a quote.

LC RNA Phosphoramidites

Item Catalog No. Pack Price ($)
10-3000-02 0.25g 75.00
10-3000-05 0.5g 150.00
10-3000-10 1.0g 275.00
10-3015-02 0.25g 40.00
10-3015-05 0.5g 80.00
10-3015-10 1.0g 160.00
10-3021-02 0.25g 75.00
10-3021-05 0.5g 150.00
10-3021-10 1.0g 275.00
10-3030-02 0.25g 40.00
10-3030-05 0.5g 80.00
10-3030-10 1.0g 160.00
Pac-A
Pac-A
Ac-C
Ac-C
iPr-Pac-G
iPr-Pac-G
U
U

LC RNA

Minimum order: 20g total Products may be combined to 20g total. Please request a quote.

UltraMild TBDMS RNA Phosphoramidites

Item Catalog No. Pack Price ($)
20-3300-01 0.1g 40.00
20-3300-02 0.25g 95.00
20-3300-10 1.0g 355.00
  1 µmole columns 20-3400-41 Pack of 4 100.00
  0.2 µmole columns 20-3400-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3400-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3400-14 Pack of 1 300.00
20-3303-01 0.1g 40.00
20-3303-02 0.25g 95.00
20-3303-10 1.0g 355.00
  1 µmole columns 20-3403-41 Pack of 4 100.00
  0.2 µmole columns 20-3403-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3403-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3403-14 Pack of 1 300.00
20-3315-01 0.1g 40.00
20-3315-02 0.25g 95.00
20-3315-10 1.0g 355.00
  1 µmole columns 20-3415-41 Pack of 4 100.00
  0.2 µmole columns 20-3415-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3415-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3415-14 Pack of 1 300.00
20-3321-01 0.1g 40.00
20-3321-02 0.25g 95.00
20-3321-10 1.0g 355.00
  1 µmole columns 20-3421-41 Pack of 4 100.00
  0.2 µmole columns 20-3421-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3421-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3421-14 Pack of 1 300.00
20-3324-01 0.1g 40.00
20-3324-02 0.25g 95.00
20-3324-10 1.0g 355.00
  1 µmole columns 20-3424-41 Pack of 4 100.00
  0.2 µmole columns 20-3424-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3424-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3424-14 Pack of 1 300.00
20-3330-01 0.1g 40.00
20-3330-02 0.25g 95.00
20-3330-10 1.0g 355.00
  1 µmole columns 20-3430-41 Pack of 4 100.00
  0.2 µmole columns 20-3430-42 Pack of 4 75.00
  10 µmole column (ABI) 20-3430-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-3430-14 Pack of 1 300.00
Pac-A-RNA-CPG
Pac-A-RNA-CPG
Bz-A-RNA-CPG
Bz-A-RNA-CPG
Ac-C-RNA-CPG
Ac-C-RNA-CPG
Ac-G-RNA-CPG
Ac-G-RNA-CPG
iPr-Pac-G-RNA-CPG
iPr-Pac-G-RNA-CPG
U-RNA-CPG
U-RNA-CPG

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

TBDMS RNA Supports

ABI-style columns are supplied for 1 µmole and 0.2 µmole scales unless otherwise requested (see note box).
Item Catalog No. Pack Price ($)
Cap Mix A
40-4210-52 200mL 140.00
  (Applied Biosystems) 40-4210-57 450mL 300.00
40-4212-52 200mL 140.00
  (Expedite) 40-4212-57 450mL 300.00
Deprotection Solution
60-4600-30 30mL 30.00

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019