ALTERNATIVE SOLVENTS/REAGENTS

Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution.
Item Catalog No. Pack Price ($)
Activator
30-3040-10 1g 35.00
30-3040-20 2g 60.00
30-3040-25 25g 500.00
  (Dissolve 1g in 31mL anhydrous acetonitrile for a 0.25M solution)
30-3140-45 45mL 40.00
  (Applied Biosystems) 30-3140-52 200mL 100.00
30-3140-57 450mL 200.00
30-3140-62 2000mL 760.00
  (Expedite) 30-3142-52 200mL 100.00
30-3140-57 450mL 200.00
30-3050-10 1g 35.00
30-3050-25 25g 500.00
  (Dissolve 1g in 34mL anhydrous acetonitrile for a 0.25M solution)
30-3150-45 45mL 40.00
  (Applied Biosystems) 30-3150-52 200mL 100.00
30-3150-57 450mL 200.00
30-3150-62 2000mL 760.00
  (Expedite) 30-3152-52 200mL 100.00
30-3150-57 450mL 200.00
30-3070-10 1g 35.00
30-3070-20 2g 60.00
30-3070-25 25g 500.00
  (Dissolve 1g in 21.3mL anhydrous acetonitrile for a 0.25M solution)
30-3170-45 45mL 40.00
  (Applied Biosystems) 30-3170-52 200mL 100.00
30-3170-57 450mL 200.00
30-3170-62 2000mL 760.00
  (Expedite) 30-3172-52 200mL 100.00
30-3170-57 450mL 200.00
30-3080-10 1g 35.00
30-3080-20 2g 60.00
30-3080-25 25g 500.00
  (Dissolve 1g in 31mL anhydrous acetonitrile for a 0.2M solution)
30-3180-45 45mL 40.00
  (Applied Biosystems) 30-3180-52 200mL 100.00
30-3180-57 450mL 200.00
30-3180-62 2000mL 760.00
  (Expedite) 30-3182-52 200mL 100.00
30-3180-57 450mL 200.00
Cap Mix A
40-4010-52 200mL 30.00
40-4010-57 450mL 72.00
40-4010-62 2000mL 325.00
40-4012-62 2000mL 275.00
Cap Mix B
40-4020-52 200mL 42.00
  (Cap B solutions containing DMAP are preferred by some researchers for preparing long oligos.)
40-4120-52 200mL 30.00
40-4120-57 450mL 72.00
40-4120-62 2000mL 325.00
40-4122-62 2000mL 325.00
Oxidizing Solution
40-4132-62 2000mL 325.00
40-4632-52 200mL 250.00
40-4632-52E 200mL 250.00
  (CSO is an alternative anhydrous Oxidizer. A minimum oxidation time of 3 minutes is required on small scales..)
Deblocking Mix
40-4040-57 450mL 36.00
40-4040-62 2000mL 144.00
  (DCA solutions are more mildly acidic than the TCA equivalents, possibly causing less depurination of dA sites.)
Deblocking Mix
40-4042-57 450mL 36.00
40-4042-62 2000mL 144.00
40-4044-54 120mL Discontinued
  (Add to 4L of DCM to make a 3% solution.)
5-Ethylthio-1H-Tetrazole
5-Ethylthio-1H-Tetrazole


4,5-Dicyanoimidazole (DCI), Crystalline
4,5-Dicyanoimidazole (DCI), Crystalline

Intellectual Property

A license may be required from Ionis Pharmaceuticals, Inc. to use DCI as an activator in oligonucleotide synthesis as claimed in U.S. Patent Numbers 6,031,092; 6,160,109 and foreign equivalents.

SMI is sold under license from Avecia Biotechnology Inc.

ABBREVIATIONS

Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
dmf = dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
I2 = Iodine
iPr = Isopropyl
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

See Also

Alternative Capping Reagent

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.
Item Catalog No. Pack Price ($)
10-4410-02 0.25g 50.00
10-4410-05 0.5g 100.00
10-4410-10 1.0g 200.00
10-4410-20 2.0g 400.00
UniCap
UniCap

Intellectual Property

10-4410 is supplied under license.


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