Duplex StabilizationEpigenetics/DNA Methylation
Serinol COT-Serinol Dabcyl Biotin Fluorescein Rhodamine Cyanine Elitechgroup Dyes and Quencher Black Hole Quencher Blackberry Rhodamine (TAMRA) Acridine DNP Cholesterol Tocopherol Stearyl Psoralen EDTA Ferrocene Methylene Blue Metal Chelates Polyaromic Hydrocarbons Puromycin Quenched Autoligation (QUAL) Probes Photo Regulation
RNA Supports For 3'-Modification TOM-Protected RNA Amidites RNA Supports For TOM RNA Synthesis TBDMS-Protected RNA Amidites HT RNA Amidites UltraMild TBDMS RNA Amidites TBDMS RNA Supports UltraMild Solvents/Reagents Minor RNA Amidites (TOM) RNA Sequence Modifier (TOM-Protected) Minor RNA Amidites (TBDMS-Protected) Minor RNA Triphosphates 2'-OMe-RNA Amidites 2'-OMe-Supports HT 2'-OMe-RNA Amidites Ultramild 2'-OME-RNA Minor 2'-OMe-RNA Amidites 2'-OMe-Thiophosphoramidites 2'-F-RNA Monomers 2'-F-Arabinonucleic Acid (2'-F-ANA)
Purification (Catalog as PDF)
Glen-Pak™ PurificationGlen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5' terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak DNA 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak DNA resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo.
Scale suggestions for the Glen-Pak DNA product line are shown below:
A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.
This booklet is available online at: http://www.glenresearch.com/Technical/GlenPak_UserGuide.pdf.
Poly-Pak™ PurificationThe use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is available online at: http://www.glenresearch.com/Technical/PolyPakBooklet.pdf.
Poly-Pak Cartridge Used Manually
Glen Gel-Pak™ DesaltingThe principle of the Glen Research gel filtration column, Glen Gel-Pak™ , is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through The resin of the column. Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.
A 61-is available online at: http://www.glenresearch.com/Technical/GlenGelPak_UserGuide.pdf.
Oligo-Affinity SupportOligo-affinity supports (OAS) should ideally be compatible with automated synthesis, should be non-friable, should not shrink or swell, and should have low non-specific binding of the proteins or DNA. On the support shown below is an Adenosine residue attached through the exocyclic amino group. In this way, synthesis progresses regularly on removal of the 5'-DMT group. However, on treatment with ammonium hydroxide, the oligo is not cleaved from the support. This matrix can then be used as an affinity support for a complementary segment of DNA or RNA. Alternatively, the complementary strand can be annealed to the support and the double stranded DNA can be used as an affinity support for purifying DNA binding proteins.
We expect that OAS PS will be used for purification of components from biological fluids.
Oligo-Affinity Support (PS)
OTHER INSTRUMENT TYPES
All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.