Purification (Catalog as PDF)

Glen-Pak Purification

Glen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5' terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak DNA 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak DNA resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo.

Scale suggestions for the Glen-Pak DNA product line are shown below:


Glen-Pak DNA Product Catalog Number Synthesis Scale Compatibility
Glen-Pak DNA 50mg Purification Cartridge 60-5000-96 0 nmole - 200 nmole
Glen-Pak DNA Purification Cartridge 60-5100-XX and 60-5200-XX 10 nmole - 1.0 µmole
Glen-Pak DNA Cartridge 3G 60-5300-01 5 µmole - 20 µmole
Glen-Pak DNA 30 mg 96-Well Plate 60-5400-01 10 nmole - 50 nmole
Glen-Pak DNA 3mg 384-Well Plate 60-5500-xx up to 20 nmoles


A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.
This booklet is available online at: http://www.glenresearch.com/Technical/GlenPak_UserGuide.pdf.

Item Catalog No. Pack Price ($)
DNA Purification Cartridges
60-5000-96 Pack of 96 415.00
60-5100-10 Pack of 10 80.00
60-5100-30 Pack of 30 200.00
60-5100-96 Pack of 96 475.00
  (For use in vacuum manifolds and high-throughput devices)
60-5200-01 Pack of 1 8.00
60-5200-10 Pack of 10 80.00
  (For use with disposable syringes)
60-5300-01 Pack of 1 150.00
60-5400-01 Pack of 1 475.00
60-5500-01 Pack of 1 675.00
60-5500-10 Pack of 10 6750.00
RNA Purification Cartridges
60-6100-10 Pack of 10 95.00
60-6100-30 Pack of 30 225.00
60-6100-96 Pack of 96 575.00
  (For use in vacuum manifolds and high-throughput devices)
60-6200-01 Pack of 1 9.50
60-6200-10 Pack of 10 95.00
  (For use with disposable syringes)
Reagents
60-4110-52 200mL 60.00
60-4110-57 450mL 120.00
60-4110-60 960mL 200.00
60-4110-62 2000mL 400.00
60-4040-57 450mL 36.00
60-4120-82 250mL 80.00
60-4120-80 1L 200.00
Racks and Seals
60-0010-01 Pack of 1 20.00
  (For use with 96 well manifolds)
60-0020-01 Pack of 1 30.00
  (For use with 96 well manifolds)
glenpaks

See Also

Poly-Pak Purification

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is available online at: http://www.glenresearch.com/Technical/PolyPakBooklet.pdf
Item Catalog No. Pack Price ($)
Packing, Cartridges and Barrels
60-1000-05 5g 70.00
60-1000-25 25g 350.00
60-1100-01 Pack of 1 8.00
60-1100-10 Pack of 10 80.00
60-3100-01 Pack of 1 12.00
60-3100-10 Pack of 10 120.00
Reagents
60-4110-52 200mL 60.00
60-4110-57 450mL 120.00
60-4110-60 960mL 200.00
60-4110-62 2000mL 400.00
60-4040-57 450mL 36.00
polypak

Poly-Pak Cartridge Used Manually

Glen Gel-Pak Desalting

The principle of the Glen Research gel filtration column, Glen Gel-Pak™ , is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through The resin of the column. Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.

There are many benefits to Glen Gel-Pak columns:

Versatility:

  • Ability to directly desalt oligonucleotides deprotected in either 30% ammonium hydroxide OR 50:50 ammonium hydroxide/40% aqueous methylamine (AMA)
  • Easily exchange buffers
  • Simple clean-up of labelling reactions
  • Mild method for purification from salts and solvents such as DMSO and DMF

Capacity:

  • Multiple column sizes (0.2 mL, 1.0 mL and 2.5 mL) are available to match synthesis scale
  • Ability to efficiently desalt short and long oligos at different scales using the same protocol
  • Suitable for oligos >10mer in length
  • Glen-Gel Pak

    Glen
    Gel-Pak 0.2
    Glen
    Gel-Pak 2.5
    Glen
    Gel-Pak 1.0


    A 61-is available online at: http://www.glenresearch.com/Technical/GlenGelPak_UserGuide.pdf.
    Item Catalog No. Pack Price ($)
    61-5002-05 Pack of 5 30.00
      (0.2 mL Capacity) 61-5002-50 Pack of 50 300.00
    61-5010-05 Pack of 5 35.00
      (1.0 mL Capacity 61-5010-50 Pack of 50 350.00
    61-5025-05 Pack of 5 45.00
      (2.5 mL Capacity) 61-5025-25 Pack of 25 225.00

    Oligo-Affinity Support

    Oligo-affinity supports (OAS) should ideally be compatible with automated synthesis, should be non-friable, should not shrink or swell, and should have low non-specific binding of the proteins or DNA. On the support shown below is an Adenosine residue attached through the exocyclic amino group. In this way, synthesis progresses regularly on removal of the 5'-DMT group. However, on treatment with ammonium hydroxide, the oligo is not cleaved from the support. This matrix can then be used as an affinity support for a complementary segment of DNA or RNA. Alternatively, the complementary strand can be annealed to the support and the double stranded DNA can be used as an affinity support for purifying DNA binding proteins.

    We expect that OAS PS will be used for purification of components from biological fluids.
    Item Catalog No. Pack Price ($)
    26-4001-01 0.1g 180.00
      (OAS PS) 26-4001-02 0.25g 425.00
    26-4001-10 1.0g 1590.00
    Oligo-Affinity Support (PS)
    26-4101-41 Pack of 4 300.00
    Oligo-Affinity Support (PS)
    Oligo-Affinity Support (PS)

    OTHER INSTRUMENT TYPES

    All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

    Monomers  

    For Instrument type
    Add
    Expedite
    E
    Mermade
    M

    Columns  

    For Instrument type
    Add
    Expedite
    E
    Applied Biosystems 3900
    A
    Mermade
    M

    Please inquire for availability of columns for other instrument types.


    Contact to place an order.
    INTERNATIONAL USERS: Contact your distributor or order from USA
    Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
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